On-Demand Webinar Streaming

timsTOF Pro and PASEF: Multiplying Sequencing Speed and Sensitivity in Proteomics

Speakers: Andreas-David Brunner, Mass Spectrometry Instrumentation,Max Planck Institute of Biochemistry; Florian Meier, Mass Spectrometry Instrumentation, Max Planck Institute of Biochemistry; Prof. Juergen Cox, Ph. D., Computational Systems, Max Planck Institute of Biochemistry

Applications of proteomics to cell biology and biomedical research require further developments of mass spectrometry (MS) technology to overcome long-standing limitations in speed, sensitivity and robustness. Trapped ion mobility spectrometry (TIMS) coupled to a quadrupole time-of-flight (QTOF) analyzer has shown much promise in this regard. Here, we further show the integration of ‘Parallel Accumulation followed by Serial Fragmentation’ (PASEF, Meier et al., J Proteome Res 2015).

In this Webinar, we will explain the fundamentals of this technology and give examples of how the timsTOF Pro was applied for high sensitivity and high speed label-free proteome quantification. We will furthermore discuss factors for the success of these experiments, starting from sample preparation and liquid chromatography to method development and data analysis with MaxQuant.

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Label-Free High-Throughput MALDI TOF Mass Spectrometry in Drug Discovery

Speaker: Matthias Trost, Professor of Proteomics, Newcastle University

The presentation will give a current overview of label-free high-throughput MALDI TOF mass spectrometry and its use in drug discovery screening. It will explain the basics of MALDI TOF mass spectrometry and the benefits of label-free screening compared to current labeled approaches. Data presented will focus on enzymes in the ubiquitin system (deubiquitylases, E2/E3 ligases) and protein kinases.

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Eliminating False Positives and Negatives: Correctly Report Results the First Time

Speaker: Dr. Carsten Baesmann, Director of Applied Markets Solutions Development, Bruker Daltonics

The webinar provides a detailed overview of how high-resolution mass spectrometry and data acquisition using a 'catch all compound' acquisition scan mode can reduce instances of reporting false positives and negatives.

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SCiLS Lab: Feature Extraction with Principal Components

Mass spectrometry imaging produces highly complex data that is difficult to access and understand. This webinar demonstrates feature extraction with principal components and advanced visualization techniques in SCiLS Lab

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Toxtyper Screening - A New Kind of Forensic Evidence

Speaker: Dr. Andrea Steuer, Forensic Toxicologist Dept. of Forensic Pharmacology & Toxicology, Institute of Forensic Medicine, Zürich

The webinar briefly introduces the field of forensic toxicology and focus on the use of the Toxtyper system for urine screening within this field.

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