Advancing Interactomics Workflows With 4D-Proteomics on the timsTOF Pro

You will hear from leaders in the field using the uncompromised scan speed, sensitivity and robustness of the timsTOF Pro mass spectrometer to elucidate protein-protein interaction networks.

Dr. André Michaelis (Professor Matthias Mann Lab at MPI) will present TIMS and PASEF® technology and its application in successfully performing over 4150 pull-downs and the completion of more than 8300 LCMS measurements in less than 20 weeks for proteome-wide interaction studies in S. cerevisiae.

Professor Peter Jackson (Stanford University) will present on how combining affinity purification mass-spectrometry (AP/MS) and shotgun proteomics with genetic interaction mapping reveals new proteins critical for KRAS-driven cancers.

• TIMS and PASEF provide near 100% duty cycle and 120Hz sequencing speed
• Robustness and throughput demonstrate the ultimate platform for affinity purification MS workflows
• How AP/MS using 4D-Protemics workflows uncover new proteins critical in KRAS cancer
• 8300 LCMS runs are accomplished in 20 weeks' time, averaging 415 LCMS runs per week (or nearly 60 runs per day)