June 6-9 | Signia by hilton orlando bonnet creek, fl

AGBT General Meeting 2022

The Preeminent Genome Science and Technology Conference

Bruker @ AGBT: Performance-Leading Innovation in Spatial and Single-Cell Omics

Driving Single-Cell and Subcellular Discovery Across the Omics Spectrum

Rapid technological advances have enabled progressively sophisticated and robust genomic, transcriptomic, and proteomic analyses. Through a range of innovative technologies, Bruker is opening new avenues of inquiry to help researchers gain greater multi-omic understanding across spatial biology applications. The Acuity Spatial Genomics product suite offers genome-wide in situ visualization of spatially resolved 3D chromatin architecture in individual cells and cell populations. Canopy Biosciences' ChipCytometry provides the ability to perform highly multiplexed targeted spatial and single-cell proteomics. Bruker's Vutara VXL Single-Molecule Localization bioimaging workstation supports flexible, multiplexed omics research beyond the diffraction limit, and the latest Bruker timsTOF SCP Mass Spectrometry instruments now enable routine ex situ unbiased proteomics of single cells.

There are a number of opportunities to meet Bruker at AGBT 2022. Register for our Tuesday evening symposium and reception (see below); visit one of our poster presentations; stop by Bruker's Hospitality Suite (Nassau Room) and hear about our cutting-edge offerings in spatial biology and single-cell omics. Bruker is pleased to showcase solutions for:

• Spatial 3D Genomics
• High-Plex Spatial Proteomics
• Single-Cell Proteomics
• Super-Resolution Omics

FIND US AT AGBT:
June 6-9, 2022
Nassau Suite
Hilton Bonnet Creek, Orlando, FL

Bruker's Hospitality Suite is in the Nassau Suite, located along the Sponsorship Promenade. | Map

Tuesday Symposium & Reception

Bruker Technology Spotlight | Cutting-Edge Solutions for Quantitative Spatial  Biology & Single-Cell Omics    

Join us Tuesday evening, 5:15 - 6:15 pm

Engage in illuminating discussion and delectable finger food as we delve into the latest technologies and research in spatial and single cell biology. Wine and light fare will be served. Please register for the event so that we may plan food and beverage accordingly.

Guest speakers:

Lixin Zhang, MD Ph.D. | Research Scientist, Magee-Womens Research Institute and Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh School of Medicine | Magee-Womens website
"Ovarian cancer: from tissue spatial identity to individual cellular activity"


Nikolai Slavov, Ph.D. | Allen Distinguished Investigator and Associate Professor, Bioengineering Department and Barnett Institute, Northeastern University | Slavov Lab
"Exploring functional protein covariation across single cells"


Bruker Technology Spotlight |
Cutting-Edge Solutions for Quantitative Spatial Biology & Single-Cell Omics
TUESDAY | 5:15 - 6:15 PM
Nassau Hospitality Suite

Have a question about the event?
Contact Sara via email: sara.tichenor@bruker.com

Many ways to connect

Find Bruker throughout AGBT

Poster Sponsor

We are proud to sponsor the Poster Session with Coffee and Dessert on Tuesday. Stop by our table outside of the poster hall to learn about Bruker and pick up some fun Bruker give-aways. 

Tuesday, June 7
1:30 - 3:00 PM
Bonnet Creek Ballroom Salon VII-XII

Technology Spotlight Symposium

Join us Tuesday evening for an hour of discussion, wine, and appetizers. This is a great opportunity to hear about our latest technologies and research in single cell and spatial omics with guest speakers. 

Tuesday, June 7
5:15 - 6:15 PM 
Nassau Hospitality Suite
Reserve a spot

CLICK2WIN Night

Let's party! Find our AGBT mascot somewhere in the suite and snap a pic with a Bruker representative! Grab a refreshment. Partake in the entertainment. Chat with us about your work and our offerings. 

Tuesday, June 7
9:30 PM - midnight

 

Bronze Sponsor Presentation

Don't miss Mark R. Munch, Ph.D., President of Bruker NANO & CEO Acuity Spatial Genomics, Inc. speak during the Bronze Sponsor Workshop.

Bruker presents novel cutting-edge solutions for deeper, quantitative analysis of spatial biology and single cell omics

Wednesday, June 8
4:15 - 4:27 PM 
Floridian Ballroom 

Posters

Bruker is pleased to have a number of posters accepted this year. Come by and talk with our scientists during poster hours:

Wednesday, June 8
4:40 - 6:10 PM

1 | Poster #110 – Thomas Campbell, Ph.D., Canopy BioSciences | Precise high-plex spatial immune cell profiling with ChipCytometry™

2 | Poster #420 – Huy Nguyen, Ph.D., Acuity Spatial Genomics | Massively multiplex single-cell 3D genome imaging with OligoFISSEQ

3 | Poster #562 – Gary Kruppa, Ph.D., Bruker Daltonics | Development of a novel TIMS-QTOF mass spectrometer for single cell proteomics

Hospitality Suite

Along the sponsorship promenade, you'll find Bruker's hospitality suite in the Nassau Suite. Swing by anytime. Visit during coffee breaks or when taking a breather from the scientific program. In the suite, you'll find information about four Bruker solutions in  spatial 3D genomics, high-plex spatial proteomics, single-cell proteomics, and super-resolution omics.

Tuesday - Thursday, June 7-9
8:00AM - 5:00 PM
Nassau Suite

Or schedule a private meeting with us at your convenience, email: sara.tichenor@bruker.com.

Bruker Posters at AGBT

Meet Our Scientists and Their Research

Poster #110 | Precise high-plex spatial immune cell profiling with ChipCytometry

Thomas Campbell, Ph.D.1 (Presenting Author)
1 Canopy Biosciences, St. Louis, MO  

Immunohistochemistry (IHC) is the most widely used diagnostic technique in tissue pathology. However, standard IHC is associated with several limitations including the labeling of a single marker per tissue section and limited quantification of cell populations. The limited multiplexing results in missed opportunities to ask key questions about tumor biology that could be important for diagnostic and prognostic outcome. ChipCytometry is a novel precise spatial multiplexing imaging platform that addresses these challenges by combining iterative immuno-fluorescent staining with high-dynamic range imaging to facilitate quantitative phenotyping with single-cell resolution. The platform enables simultaneous detection of multiple markers on a single tissue section and enable accurate quantification of protein expression levels are necessary to deeply profile single cells, understand interactions between key immune cells, and identify topographic biomarkers. Standard FCS files are generated from multichannel OME-TIFF images, enabling identification of cellular phenotypes via flow cytometry-like hierarchical gating. Quantification of results reveal precise expression levels for each marker in the assay in each individual cell in the sample, while maintaining spatial information about each cell. ChipCytometry has the potential to inform the discovery of novel biomarkers for immuno-oncology research and is likely to have a significant impact on precision medicine and human health in the future.

Poster #420 | Massively multiplex single-cell 3D genome imaging with OligoFISSEQ

Huy Q. Nguyen, Ph.D.1 and Shyamtanu Chattoraj, Ph.D.1
1 Acuity Spatial Genomics, Newton, MA  

Recent advances in genome imaging and next-generation sequencing are revealing the incredible complexity and biological importance of the genome’s 3D configuration. Still, many questions remain: How does 3D genome organization relate to normal health and disease? How are chromosomes organized in aneuploidies? Does having an extra copy of one chromosome affect the others? Being able to answer these questions requires a complete spatial map of the genome. Unfortunately, directly imaging multiple loci (>5) to visualize genome 3D organization within large numbers of single nuclei has been challenging.

Towards the goal of directly visualizing entire genomes at high resolution, we developed OligoFISSEQ1. OligoFISSEQ combines Oligopaint technology for “painting” loci with complex libraries of inexpensively synthesized oligonucleotides and the multiplexing power of fluorescent in situ sequencing (FISSEQ) to simultaneously target, visualize, and localize any number of genomic loci. In particular, the OligoFISSEQ suite of methods involve hybridizing barcoded in situ hybridization probes (Oligopaints) to chromatin targets, followed by in situ barcode detection by FISSEQ.

Here, we will describe OligoFISSEQ and its application for exploring genome organization. We will show significant improvements that we have made to the published work to increase scalability in detecting higher number of targets across the genome in single cells making it a powerful tool to trace targeted genomic structures.  We recapitulate known characteristics of genome organization, such as chromosome territories, thus, validating our technology. Additionally, we use OligoFISSEQ to trace individual chromosomes at high genomic resolution. Hierarchical clustering of these individual chromosome traces allowed identification of subgroups of cells exhibiting specific chromosome structures. To demonstrate OligoFISSEQ sensitivity, we apply OligoFISSEQ to a variety of diseased and non-diseased cell and tissue types to identify differences in genome organization. Being able to obtain population averaged data while maintaining single cell resolution is essential for identifying problematic cells within a tissue and will further elucidate the links between genome organization and disease.  

1 Nguyen HQ, Chattoraj S, Castillo D, Nguyen SC, Nir G, Lioutas A, Hershberg EA, Martins NMC, Reginato PL, Hannan M, Beliveau BJ, Church GM, Daugharthy ER, Marti-Renom MA, Wu CT. 3D mapping and accelerated super-resolution imaging of the human genome using in situ sequencing. Nat Methods. 2020 Aug;17(8):822-832

Poster #562 | Development of a novel TIMS-QTOF mass spectrometer for single cell proteomics

Gary Kruppa1, Kristina Marx1, Renata Blatnik1, Verena T1, Markus L1, Oliver R1, Nagarjuna N1
Bruker Daltonik, GmbH, Bremen, Germany  

Introduction
Single cell proteomics is a young niche of proteomics compared to single cell genomics. In recent years, significant progress has been made toward single cell proteomics through improvements in sample handling, and boosting the sample signal by multiplexing, but coupled to existing mass spectrometry technology. The timsTOF SCP is the first commercially introduced mass spectrometer dedicated for single cell proteomics driven by a substantial boost in sensitivity. The modified front-end of the instrument increases the sensitivity by 4-5 fold ion transfer into the mass spectrometer. Here we demonstrate the performance of the instrument for low sample loads, equivalent to a single cell in combination with robust low flow rate nanoflow LC sample introduction.
Methods
Commercially available K562 tryptic peptide digests (Promega) were onto the LC sample introduction system. Peptide amounts of 250 pg, 500 pg, 1 ng and 10 ng were used to evaluate the performance of the instrument. A low flow method (100 nl/min) and a gradient time of about 28 minutes was used for separating the eluting peptides on a 15 cm 75 micron ID column coupled to captive spray ionization source using a 10-micron ID zero-dead volume emitter. Samples were analyzed using a data independent analysis method (dia-PASEF) tuned for high sensitivity measurements. Raw data were processed in Spectronaut 15 (Biognosys) or DiaNN.
Preliminary data
Using the combination of low flow nanoLC and the high sensitivity data acquisition methods on the timsTOF SCP, we could quantify above 1000 protein groups from 250 pg sample loads and close to 2000 protein groups from 500 pg loads. Performing a dilution series experiments, more than 4000 protein groups could be quantified in 6.4 ng peptide loads. We plan to further increase the depth of proteome quantification by increasing the depth of the library used in the data processing step. Further we plan to investigate the stability and the effect of sample storage of such low sample amounts on the depth of proteome quantification.

About AGBT

Advances in Genome Biology and Technology is considered the preeminent genome science and technology conference where top global researchers, leaders and innovators meet to announce new discoveries, cutting edge breakthroughs and to collaborate. As a recognized cornerstone for the genomics research community, AGBT provides an outstanding forum for exchanging information about the latest advances in DNA sequencing technologies, experimental and analytical approaches for genomic studies, and their myriad applications. The meeting format includes daytime plenary sessions that feature invited speakers and abstract-selected talks that highlight cutting-edge research across the broad landscape of genomics. The evening concurrent session includes experimental and computational approaches for effectively utilizing the latest DNA sequencing technologies.

CONFERENCE VENUE:
Signia by Hilton Orlando Bonnet Creek, Orlando, FL
14100 Bonnet Creek Resort Lane | Map


HEALTH AND SAFETY:

AGBT is requiring proof of vaccination or a negative PCR test within 48 hours of the event.
AGBT.org