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nanoElute® - Simply Connect

Modern HRAM nano-ESI MS analysis is putting increased pressure on the front-end LC separation to match the MS in terms of robustness and reliability as well as demanding the absolute highest quality and reproducibility of sample separation.

To ensure such individual system performance as well as consistency over multiple systems and/or labs, deeper system integration and novel approaches to quality control as well as intelligent system diagnostics are called for. This in turns requires rigorous protocols and automation for all phases of the experiment, driving new approaches to user interface design and data monitoring, as well as a critical rethink of all flow paths and liquid handling parts of the configuration.

The Bruker nanoElute is designed with years of proteomics know-how and nano-UHPLC expertise.
 
Based on industry leading components coupled with innovative solutions to common problems, it delivers trouble-free operation from the moment of installation through years of OMICS knowledge generation.

Dr leonard forster

Professor Leonard Foster Director, Centre for High-Throughput Biology University of British Columbia, Canada:

"LC-MS experts and biologists alike require trouble-free operation and ease-of-use along with top performance. The integrated nanoElute system readily delivers through an intuitive, wizard-driven interface with intelligent diagnostics and built-in trouble-shooting."

nanoElute - the perfect companion

When paired with the ultra high-resolution / accurate mass QqTOF, the Bruker impact II and the market proven CaptiveSpray ionization source, the nanoElute System is unbeatable when it comes to:

 

 

nanoElute paired with impact II and CaptiveSpray ionization source.
The ultra high-resolution, accurate mass QqTOF, the Bruker impact II and the market proven CaptiveSpray ionization source are the perfect companions for the nanoElute system.

The complexity of the nanoElute’s built-in intelligence and applied chromatographic understanding is hidden behind a user interface that is intuitive and easy to use, but also powerful and flexible to operate.

 

Simply Connect.

nanoElute® - Intuitive Innovation

Designed with industry leading components

The main performance characteristics of its novel system configuration and deep software integration are:

  • Split-free flow from single stroke piston pumps (1300µl)
  • Seamless 1 and 2 column operation
  • Pressure range 0-1000bar
  • Built-in quality control and scheduling of preventive maintenance
  • Gradient flow range: 50-2000nl/min with inline flow sensors
nanoElute
The complexity of the nanoElute’s built-in intelligence and applied chromatographic understanding is hidden behind a user interface that is intuitive and easy to use, but also powerful and flexible to operate.

Maintaining premium LCMS data quality is dependent upon having a reliable and robust separation system. One of the greatest challenges working with nanoflow UHPLC systems is to maintain a leak tight system at UHPLC pressure. Even nanoflow leak rates can cause significant shifts in retention time and disturb the analysis.

The system performance is maintained through 3 layers of protection:

  1. Automatic initialization, where the autosampler and pumps automatically run a preparation protocol before sample injection ensuring performance from the first to the last sample.
  2. Built-in scheduling of preventive maintenance jobs based on usage patterns and integrated system expertise where the software will suggest how to optimize continued system operation.
  3. Precise system diagnostics that automatically detects leaks and blockages and highlights them in the graphical user interface.

Tailor-made instrument methods are automatically generated using the Instant Expertise™ method editor, based on experimental mode, column selection and analysis time, delivering:

  • LC-MS/MS method with intelligent, self-optimizing LC separation for higher productivity and expert-caliber results the first time.
  • Optimal experimental conditions are automatically calculated from column type and sample characteristics to suit all proteomics samples
  • Chromatographic resolution is preserved for best simultaneous ID and Quant capabilities

Gradients can be readily modified but most other parameters are hidden although they can still be edited in “expert” mode.

The system is preconfigured with a standard layout and a novel valve solution to ensure ease-of-use and system stability. The unique valve design ensures that the user can execute three different types of experiments without hardware changes and software controlled flow paths exist for using a trap column, bypassing the trap column or doing direct infusion for fast analyses of simple samples

The unique valve design and novel instrument configuration enable:

  • Software controlled flow paths
  • Dynamic inclusion of trap column
  • Backflushing the sample for sharper peaks


New nano-columns and a unique back-flushable trap cartridge holder complement the instrument and are recognized by the Instant Expertise algoritm.

Compact and dedicated design with market proven autosampler and large volume, single stroke piston pumps support both:

  • Longer gradients
  • Wide flow range for efficient OMICS knowledge Generation

Robust and accurate gradient delivery with intelligent diagnostics

Trouble-free OMICS knowledge generation

The nanoElute system provides excellent chromatographic performance even for very short gradients in both separation modes. With a trap column installed, the focus is on speed and robustness, however the chromatographic performance is maintained due to new nano-columns and unique back-flushable trap cartridge.

With trap column

Without a trap column installed, ultimate separation power is delivered where the trap column is elegantly by-passed by virtue of the novel valve geometry.

Without column trap

The unique instrument configuration allows the user to analyze samples in both “with trap” and “without trap” mode in the same sample batch without physically changing the hardware setup.

Separation of complex samples with a long column and gradient has been demonstrated both with and without trap column. The retention time and area reproducibility is excellent displaying suitability for all proteomics applications, including the most demanding ones.

Method

Predigested Bovine Serum Albumin (Bruker, Billerica, MA) was used as a representative for a simple proteomics sample (25 fmol/µl).
HeLa cell lysate was prepared according to Beck et al.¹ and acidified with formic acid to a final concentration of 200ng/µl. For the simple sample, the tryptic peptides were separated on a novel nano-UHPLC system using a 10min reversed phase gradient (2% - 35% solvent B in 10min. - 0.1% FA in ACN), on standardized 15cm 75µm bore column with C18 resin. The HeLa sample was separated with a reversed phase gradient (from 2% -24% in 90min, 24-48 in 10min. solvent B - 0.1% FA in ACN), on a standardized 40cm 75µm bore columns with C18 resin. Data was acquired with an impact II QTOF MS (Bruker Daltonik, Bremen, Germany) equipped with a CaptiveSpray ion source.

References:
[1] Beck, S., Michalski, A., Raether, O, Lubeck, M., Kaspar, S., Goedecke, N., Baessmann, C., Hornburg, D, Meier, F., Paron, I., Kulak, N.A., Cox, J. and Mann, M. “The Impact II, a Very High-Resolution Quadrupole Time-of-Flight Instrument (QTOF) for Deep Shotgun Proteomics.” Mol Cell Proteomics. 2015 Jul;14(7):2014-29.

Direct infusion for fast analysis

Direct Infusion for fast analysis

The unique valve design also allows ”Direct Infusion” for fast runs of simple samples e.g. BSA at 2 μl/min.

Bruker Daltonics services are performed by factory trained and certified engineers. DIN EN ISO 9001:2000 requirements are fulfilled. 

 

Available services include:

  • Help desk service for a faster access to our support team, to your service order follow up and for general information
  • Planned maintenance, according to our requirement
  • Telephone support from highly-skilled troubleshooting professionals, to isolate and resolve hard- and software problems
  • Web-based remote instrument service for service diagnosis and applications support
  • Customer on-site repair and maintenance service
  • Spare parts availability typically over night or within a few working days worldwide
  • Compliance services for installation qualification, operational qualification / performance verification
  • Site planning and relocation
  • Advise to improve labs efficiency (consumable, method development & how to run the complete solution)

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.