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Combining X-Omics performance with high MALDI sensitivity

timsTOF fleX uniquely enables SpatialOMx

Whether it’s proteomics, lipidomics, glycomics, or metabolomics, the timsTOF fleX is the ideal platform for deep SpatialOMx analyses. Use patented SmartBeam™ 3D technology to conduct fast, label-free MALDI Imaging for mapping the distribution of molecules in your sample and identify regions of interest for more specific analyses. Apply PASEF powered LC-MS/MS for the highest level of identifications and for greatest confidence in your results.

Differences in organ functions are reflected in differences in their expressed proteins
Differences in organ functions are reflected in differences in their expressed proteins

High speed and high sensitivity for biomarker discovery

Combining the power of trapped ion mobility spectrometry (TIMS) and Parallel Accumulation Serial Fragmentation (PASEF), the timsTOF fleX can deliver quantitative information from smaller amounts of highly complex mixtures. Using less than 200 ng sample load and a 90 min gradient length, more than 5400 protein groups can be identified from a typical human cell line lysate. The high sensitivity reduces sample preparation costs and decreases time lost to maintenance.

MALDI Imaging for investigating compound distributions

With existing protocols for lipidomics, metabolomics or glycomics, MALDI Imaging spectra can be used as a molecular fingerprint for specific regions, or to investigate how compounds are localized across an entire sample for biomarker discovery. This can also be used for investigation of temporal changes, quantification, and correlation of compounds to tissue states. The timsTOF fleX allows for fast high mass resolution MALDI imaging – with the SmartBeam 3D and specialized MALDI source and stage, a high spatial resolution measurement of lipids from a rat brain section consisting of 370,000 pixels takes less than 5 hours.

MALDI guided SpatialOMx for laser precise efficiency

Traditional workflows that sample small tissue sections (<100 µM) provide deep molecular details for a variety of chemical classes including RNA/DNA, proteins, glycans, lipids and metabolites. However, processing times are long and tissue excise locations are determined with optical images that lack molecular signal information. SpatialOMx on the timsTOF fleX rapidly provides a high definition, label-free overview that merges chemical information with vital morphological context. Choose your regions of interest manually or use SCiLS enabled automated segmentation to know exactly where your extraction will have the highest impact.

Introducing an automated molecular annotation workflow for SpatialOMx

Using Bruker’s industry leading software, it is now possible to annotate target molecules directly from tissue. Simply load your data into SCiLS Lab, define regions of interest, and export the peak lists to MetaboScape. Annotate your peaks using databases or compound lists from LC-MS/MS analyses, then review and export your annotations back to SCiLS for visualization. From SCiLS you can then visualize results with pathways and familiar nomenclature rather than mass labels to reduce the time from data to results. 

Figure 1
Novel Mass Spectrometry Imaging Workflow: Automated annotation of metabolites and lipids from tissue.
*Lipid Maps and HMDB are not Bruker products.

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.