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Microfluidics Accessory for Sequential Labelling in Localization Microscopy Applications

Applications are developing in localization microscopy where the number of targets for imaging far outstrips the number of probes that can be chromatically separated. Sequential labelling with microfluidics allows a much larger number of targets to be addressed.

The Bruker microfluidics accessory is a unit designed to allow sequential labelling to be incorporated into localization microscopy protocols with the Vutara 352 single molecule localization microscope. The unit is controlled by the Vutara SRX software and allows flexible configuration of fluidics sequences that are run in conjunction with imaging cycles to allow users to label an unlimited number of probes and image them in a sequential fashion.

Microfluidics Accessory

Applications

The Bruker microfluidics unit can be used with Vutara to address any localization microscopy application where a large number of targets needs to be imaged. Fifteen probe vials are provided, and the system allows probe vials to be interchanged during experiments, thus allowing an unlimited number of targets.   

Example localization microscopy applications:

  • OligoSTORM
  • DNA PAINT
  • Multiplexed antibody labelling
  • Refresh STORM

The Bruker microfluidics unit is also compatible with the Opterra confocal for serial labelling confocal applications.

Fluidics Manager

Software

Control of the unit is via SRX software.  The software has a flexible interface module (Fluidics Manager) for the set-up of fluidics.  An unlimited number of sequences can be set up, each with its own unique steps.

Informatics

The probe associated with each sequence has a specific color that is user-defined, and that is used as a color scale for visualization of experiments. During analysis with SRX, individual probes can be analyzed, or analysis can be carried out that compares probes.

Maternal and paternal

Specifications

Dimensions: 370mm x 300mm x 204mm

Reservoirs: 15 2.0 ml Eppendorf tubes for probes

Wash/buffer reservoirs: Two 15 ml, one 50 ml, one 100 ml

Flow rates: 1 ul/min – 1000 ul/min

Pumping method: Air pressure (self-contained pump)

Interchangeability:  Reservoirs can be changed out while system is in use for an experiment; allows an unlimited number of probes to be used.

Dead volume: Fluidics unit: 85 ul for probe channels, 190 ul for wash/buffer channels

Tubing from fluidics unit to flow chamber: 2 ul/cm

Flow cell: 150 ul for standard flow cell