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Utlima In Vitro Applications

Tissue Explants

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AJ Walsh, RS Cook, ME Sanders, L Aurisicchio, G Ciliberto, CL Arteaga, and MC Skala. Quantitative optical imaging of primary tumor organoid metabolism predicts drug response in breast cancer. Cancer Research (2014). Sep 15;74(18):5184-5194.

Tissue explants provide a higher throughput model for studying tissue response to pharmaceutical agents. The FLIM option allows measurement of metabolic status through measurement of fluorescent lifetimes.

High-Resolution Multifield Imaging

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5x5 montage at 40x (covering area of 1175 um x 1175 um) of mouse kidney section with Alexa Fluor® 488 WGA, Alexa Fluor® 568 Phalloidin.

The Atlas Imaging module in Prairie View software simplifies setup of 2-D and 3-D automated montages. X,Y boundaries are easily setup through a thumbnail display. Tiles not containing areas of interest can be turned off, and the Z range for 3-D montages can be variable, allowing more efficient collection of data.

Neuronal Signaling in Brain Slices

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EPSP recorded in the dendritic trunk in response to a single, high-power ungaging event at s1 (black) or s2 (grey) in brain slice; Mark T. Harnett et al., Nature 491, 599-602.

Ultima In Vitro is the system of choice for studying neuronal signaling at the both the dendritic and neural network levels. When equipped with optional photostimulation galvanometers, Ultima In Vitro provides a complete optical workstation, allowing simultaneous imaging, photostimulation  and electrophysiology recording.

Volumetric Imaging of Cell Preparations

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Tumor Cells (red) in collagen matrix (green); Courtesy Szulczewski J, University of Wisconsin Madison

Ultima In Vitro provides high resolution and efficient volumetric imaging of cell cultures and cells in artificial matrices.