Ultima IntraVital Interneurons

Utlima IntraVital Applications

Intravital Imaging with Behavioral Paradigms

Video courtesy of Michael Goard, MIT

Intravital imaging using functional indicators provides the opportunity to measure neural activity in awake, behaving specimens. Ultima IntraVital provides the imaging speed to capture activity of neural networks in single or multiple focal planes.

The unique motorized orbital nose piece enables off-axis imaging to provide access to areas of interest in specimens in a fixed orientation. Off-axis imaging also reduces scatter in areas containing highly oriented structures such as the cortex and hippocampus. Prairie View software allows Ultima IntraVital to control external stimuli and to synchronize with external systems controlling behavioral trials.

Optogenetics

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GCaMP6 and C1V1-2A-mCherry labeling, Packer et al., Nature Methods, (2014) doi:10.1038/nmeth.3217.

Optogenetics provides researchers with a set of tools for precise light induced activation of specific cells. The addition of a second set of galvanometers to Ultima Intravital allows simultaneous imaging and optogenetic activation, including the use of channel proteins such as channel rhodopsin. A variety of scan patterns are available for optogenetic activation , including points, high-speed spirals, and user-defined masks. Activation for optogentics can be synchronized with imaging or with external stimuli.

High-Resolution Anatomic Mapping of Neural Networks

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Multifield montage of neural network; Courtesy Datta SR, Harvard University.

The Atlas Imaging module in Bruker's Prairie View software simplifies setup of 2-D and 3-D automated montages. X,Y boundaries are easily setup through a thumbnail display. Tiles not containing areas of interest can be turned off, and the Z range for 3-D montages can be variable, allowing more efficient collection of data.