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Utlima Investigator Applications

Intravital Imaging with Behavioral Paradigms

Video courtesy of Michael Goard, MIT

Intravital imaging using functional indicators provides the opportunity to measure neural activity in awake, behaving specimens. Ultima IntraVital provides the imaging speed to capture activity of neural networks in single or multiple focal planes.

The unique motorized orbital nose piece enables off-axis imaging to provide access to areas of interest in specimens in a fixed orientation. Off-axis imaging also reduces scatter in areas containing highly oriented structures such as the cortex and hippocampus. Prairie View software allows Ultima IntraVital to control external stimuli and to synchronize with external systems controlling behavioral trials.


IntraVital Tumor Progression

Multiphoton intravital imaging provides a high resolution method for imaging tumor implants. Tumor progression and response to pharmaceuticals as well as immune cell response, can be measured through longitudinal imaging sessions. Ultima IntraVital can be configured to image the collagen fibers.


HIV-infected T-cells (EGFP), with uninfected T-cells (CellTrackerOrange), collagen (blue), and tissue auto-fluorescence (white); Murooka TT et al, Nature 490: 283-7, 2012.

The high-speed, volumetric imaging capability of Ultima IntraVital provides and ideal tool for studying immune cell activity in lymph nodes in vivo. 

High-Resolution Anatomic Mapping of Neural Networks

Multifield montage of neural network; Courtesy Datta SR, Harvard University.

The Atlas Imaging module in Bruker's Prairie View software simplifies setup of 2-D and 3-D automated montages. X,Y boundaries are easily setup through a thumbnail display. Tiles not containing areas of interest can be turned off, and the Z range for 3-D montages can be variable, allowing more efficient collection of data.