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Elute UHPLC – Settings standards in Retention time stability

Exceeding the guidelines for Multiresidue Screening

Today’s guidelines defining retention time (RT) deviations for pesticide screening are setting demanding new levels for the respective LC-MS methods. The new EU SANTE guidelines 11945 I 2015, require retention time deviations to meet < 0.1 min. Based on its innovative pump design the Elute UHPLC performs easily below this RT threshold by providing typical RT deviations of 0.01 min.

Multitarget Screening
Pesticide Screening of > 500 pesticides in food matrix separated on an Intensity Solo C18 RP column on the Elute UHPLC coupled to an impact II QTOF Instrument.

Ultrafast quantitative LC-MS/MS methods

Fast confirmation and quantitation of urine alcohol metabolite markers are needed to ensure high productivity for the forensic lab. These methods are routinely established by LC-TQ MS/MS methods. The Elute UHPLC coupled to the EVOQ LC-TQ enables fast and reliable confirmation and quantitation of Ethylglucoronide (EtG) and Ethylsulfate (EtS) in urine matrix.

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A fast three minute method to quantify EtG and EtS in urine with the new Bruker Elute UHPLC coupled to the EVOQ Elite triple quadrupole system.

Sensitivity for quantitation in food matrices

The minimum required performance level (MRPL) for Chloramphenicol detection in food is 0.3 ppb (EU decision 2003/181/EC). The Elute UHPLC coupled to the EVOQ Elite LC-TQ delivers great sensitivity (LLOQ 0.02 ppb) and ultra-sharp peak widths typically in the range of 2 sec. These levels can be achieved even in challenging matrices such as eggs.

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Shown is the chromatographic peak width at half maximum (2.1 – 2.4 seconds range) for Chloramphenicol in egg matrix.

On-line sample preparation

On-line liquid extraction (OLE) has major advantages compared to off-line solid phase extraction (SPE) such as: direct injection of 100% of the sample in a closed automated system and using smaller particle sizes, leads to sharper focused chromatographic peaks. The Elute OLE system combines the advantages of an UHPLC system with the process advantages of OLE improving the quality and reproducibility of your results. For demanding sample preparation tasks e.g. for water analysis or analysis of complex matrices such as urine, the pre-enrichment capability of the Elute OLE delivers an added high value benefit to your laboratory.

Pestidcides in water
Pesticides in water (600 μl vol.), Triazophos 0.5 - 1000 ppt extracted and separated by an Elute OLE
system coupled to an impact II QTOF instrument.