timsTOF Pro

timsTOF Pro – The only mass spectrometer with PASEF technology

Outstanding hardware performance

The second generation dual TIMS analyzer is optimized for shotgun proteomics needs. Due to its unique geometry ions are released dependent on their mobility from the second section of the TIMS analyzer while the further incoming ions can be accumulated in parallel in the first part of the TIMS analyzer. With the parallel accumulation technology a duty cycle up to 100% can be achieved resulting in no ion loss.

In the timsTOF Pro an advanced segmented quadrupole mass filter is used for high ion transmission and isolation efficiency. The quadrupole mass position is synchronized with the elution times of the specific ions from the TIMS analyzer. Due to its ultra-high mass position switching time (< 1 ms) it enables the best performance for the PASEF method.

Schematic of the timsTOF Pro
Schematic of the timsTOF Pro


Trapped Ion Mobility Spectrometry (TIMS) is first and foremost a separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization.


With speed in mind, Bruker experts redesigned MS/MS technology to meet the requirements of shotgun proteomics. Peptide ions are separated using trapped ion mobility spectrometry, eluted (~ 100 ms) and detected in the QTOF, generating the TIMS MS heat map (A). In the PASEF method (B) the same TIMS separation is used with the quadrupole isolating a certain ion species during its elution and immediately shifting to the next precursor. Parent and fragment spectra are aligned by mobility values. With the Parallel Accumulation Serial Fragmentation (PASEF) Technology >100 Hz sequencing speed can be achieved. With the PASEF method the MS/MS spectra quality of the low abundant peptides can be increased by selecting them several times.

The principle of PASEF acquisition
Principle of the PASEF acquisition mode
PASEF cycle time
Parallel Accumulation Serial Fragmentation (PASEF) cycle time: By synchronizing the quadrupole with the TIMS elution time, an average of 12 precursors can be fragmented within a 100 ms timescale. Intelligent software targets low-level precursors multiple times for PASEF MS/MS fragmentation.

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