TASQ software enables forensic scientists to rapidly identify passed/failed samples using an intuitive color coded results table.
ToxScreener

TargetScreener-Broadband CID (bbCID) Data Acquisition

Reliance on precursor accurate mass and retention time alone can lead to a high number of false positive results.

 

However by using Broadband CID (bbCID) Data Acquisition to generate fragment qualifier ions  throughout  the  experiment, false positive detections are practically eliminated, delivering maximum confidence in your screening result.

 

 

Illustrating the accumulation of retention time and qualifier ion information to reduce false positive detections
Illustrating the accumulation of retention time and qualifier ion information to reduce false positive detections (click to enlarge)
Bruker broadbandCID bbCID data acquisition
Schematic of Bruker broadbandCID (bbCID) data acquisition: The QTOF mass spectrometer rapidly alternates between two full scan accurate mass channels, one at low collision energy and the other at elevated collision energy (click to enlarge)

The ToxScreener continuously generates fragment ions using broadband Collision Induced Dissociation (bbCID).

 

During the screening process, the QTOF mass spectrometer rapidly and continuously cycles between low collision energy (TOF-MS) and elevated collision energy (bbCID) MS/MS, resulting in the seamless collection of accurate mass precursor and fragment ion data in a single analysis.

ToxScreener detects 'all of the ions, all of the time' in full scan MS and MS/MS modes without compromising sensitivity or resolution, results in a dataset that is complete and unrestricted. The diagnostic Qualifier Ions (QI's) can then be used as a part of an enhanced confirmation criterion to eliminate false positive findings as well as delivering unambiguous ID's for co-eluting isobaric drugs.

 

 

Eliminate False Positive Findings

and Achieve Unambiguous ID

At the heart of the ToxScreener solution is the exact mass pesticide database. In addition to monoisotopic precursor ion masses and retention time data, the database also contains diagnostic QI information, comprising of accurate mass bbCID fragments and 'in source' fragment ions as well as adduct and isomer information.

Snapshot of the ToxScreener exact mass and retention time database (click to enlarge)
Snapshot of the ToxScreener exact mass and retention time database (click to enlarge)

Intelligent Data Review

Data processing for screening and quantitation is powered by TASQ (Target Analysis for Screening and Quantitation) software. TASQ intelligently uses the diagnostic qualifier ions to reduce false positive detections, bringing a new level of confidence to screening workflows.


For total confidence in the results, four dimensions of confirmation are used in the ToxScreener 2.1 workflow

  1. Parent ion or adduct exact mass
  2. Retention time
  3. Isotopic pattern fidelity
  4. Exact mass of fragment qualifier ions


Color coded screening results are easily reviewed in either a sample centric or analyte centric fashion as desired by the analyst. Once reviewed, qualitative and quantitative sample reports are then easily generated.

Bruker ToxScreener is a ready to go solution, requiring no method development, delivering maximum productivity and confidence to your laboratory in the shortest possible time.

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