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EXSY spectrum

SECSY and EXSY

Spin Echo Correlation Spectroscopy (SECSY) and Exchange Spectroscopy (EXSY) are 2-dimensional techniques.

Spin Echo Correlation Spectroscopy (SECSY) and Exchange Spectroscopy (EXSY) are 2-dimensional techniques. The positions of cross peaks in the 2D spectrum are used to correlate spectral peaks with each other and the cross peak amplitudes carry the information about exchange rates. These experiments are standard features of the E 580 spectrometer and are supported by predefined PulseSPEL programs.

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ESE detected field sweep spectrum

Laser Triggered Experiments

An external event, e.g. a laser flash, can be used to generate a paramagnetic state.

An external event, e.g. a laser flash, can be used to generate a paramagnetic state. For this type of experiment the instrument can be triggered externally or alternatively the instrument can send a trigger pulse to activate an external device. Precise synchronization between the external event, the microwave pulses and the data acquisition is established. Any pulse-EPR experiment can be run in combination with the external trigger function.

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DEER time domain signal

Pulse ELDOR and DEER

With this technique the dipolar interaction between electron spins is measured.

Electron-Electron Double Resonance (ELDOR) and Double Electron-Electron Resonance (DEER) are acronyms for the same technique. With this technique the dipolar interaction between electron spins is measured. This information directly translates into the distance between the electron spins. Distance ranges of 10 to 80 Angstrom are covered. In recent years the combination of site directed spin labeling and DEER became one of the most popular tools in structural protein research.

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2D-HYSCORE spectrum

ESEEM and 2D-HYSCORE

Electron Spin Echo Envelope Modulation (ESEEM) is one of the most popular techniques to measure the hyperfine interaction (HFI) parameters between electron spins and nearby nuclear spins.

Electron Spin Echo Envelope Modulation (ESEEM) is one of the most popular techniques to measure the hyperfine interaction (HFI) parameters between electron spins and nearby nuclear spins. From the analysis of the ESEEM signals detailed information about electron spin density distribution, distances and bonding angles is gained. 2D-HYSCORE is a technique also based on the ESEEM effect and is the method of choice for frozen solutions and powders. All ESEEM-type of experiments are standard features of the E 580 and are supported by dedicated acquisition and processing tools in Xepr.

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