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NUS removes a time barrier for high-resolution multidimensional NMR spectroscopy and is especially useful for multi-dimensional experiments in biomolecular NMR, where time savings up to a factor of 4 in 3D experiments or 10 and even more in 4D and 5D experiments can be achieved. With NUS, new protein structure determination experiments now become feasible. 

Small molecule applications based on 2D spectra can also benefit from an accelerated acquisition by a factor of 2. Of special interest here are improvements in spectral resolution and quality delivered by NUS without the need to increase the overall acquisition time. 

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