AFM PeakForce Tapping Gallery

Resolving Submolecular Detail in a Monolayer of Oligo(Para-Phenylene)

Resolving submolecular detail in a monolayer of oligo(para-phenylene) shows how alkyl-chain packing drives self-assembly. 20nm image with 0.2nm Z-scale. Using PeakForce Tapping on Dimension Icon. Courtesy of Wael Hourani, Universite Bourgogne Franche-Comte, CNRS, France.

Height and Stiffness Maps of Calcite

Height (A) and stiffness (B) maps of calcite. This unique atomic scale nanomechanical information reveals increased contact stiffness for alternate rows of atoms.

ScanAsyst Probe Capabilities

Deep (~65nm), narrow (<50nm) trenches are a very challenging sample for TappingMode. ScanAsyst, however, permits the probe to travel at the bottom of narrow trenches. Scan size 1μm.

PeakForce QNM -ULDPE / PS-LDPE sealant layer

This modulus image shows the subtle transition between the ULDPE tie layer to the PS/LDPE sealant layer of cross sectioned packaging material. Mapping the modulus at high spatial resolution reveals lamella from the tie layer act as nucleation sites penetrating into the sealant and increasing lamellar ordering within 1 ?m from the interface. Image size 3 ?m.

Anti-Bacterial Film Consisting of Poly(methacrylate)

Anti-bacterial film consisting of poly(methyl methacrylate) and silver nanoparticles. Sample was imaged on a Dimension Icon using PeakForce QNM at a scan size of 13.5 µm. The data shown is adhesion data overlaid on topography. (Sample courtesy of Mishae Khan and Daniel Bubb, Rutgers University)

Arabidopsis Thaliana Shoot Apical Meristem

Projection from a confocal stack of an Arabidopsis Thaliana shoot apical meristem. White rectangle shows location of anticlinal cell walls and AFM image area for topography and DMT modulus images. (Image courtesy of Pascale Milani, Arezki Boudaoud, and Olivier Hamant (Ecole Normale Superieure de Lyon, France))

Arabidopsis Thaliana Shoot Apical Meristem - DMT Modulus

DMT modulus image of Arabidopsis Thaliana shoot apical meristem labeled with FM4-64. Sample imaged with BioScope Catalyst using PeakForce QNM. (Image courtesy of Pascale Milani, Arezki Boudaoud, and Olivier Hamant (Ecole Normale Superieure de Lyon, France))

Arabidopsis Thaliana Shoot Apical Meristem - Topography

PeakForce QNM topography image of Arabidopsis Thaliana shoot apical meristem. Imaged with BioScope Catalyst. (Image courtesy of Pascale Milani, Arezki Boudaoud, and Olivier Hamant (Ecole Normale Superieure de Lyon, France))

Calcite in fluid

Atomic-resolution imaging of calcite in fluid using PeakForce Tapping on the Dimension FastScan.

IPMMA Adhesion

Submolecular resolution adhesion maps of isotactic poly(methyl methacrylate) acquired with Dimension FastScan using PeakForce QNM. Image width 50nm. (Sample courtesy of Prof. Dr. Thurn-Albrecht, Martin-Luther-Universitat Halle-Wittenberg)

Glioblastoma Cancer Cell Cytoskeleton - Deformation (E)

PeakForce QNM investigation of live U-251 Glioblastoma cells. 80x80µm 128x128 deformation image of cells transfected to overexpress TSF.

Mellanin-butterfly wing

PeakForce QNM image of melanin layer under a scale on a butterfly wing (500nm scan). Quantitative modulus data has been overlaid on a 3D representation of the surface topography, making it easy to relate the mechanical properties to their corresponding structures.

Pentacene Layers

MultiMode 8 image of pentacene layers grown on an HMDS-treated silicon oxide surface. Each layer is 1.7nm thick, corresponding to the width of a single entacene molecule. The 2µm scan area was imaged at 6Hz with ScanAsyst-HR. (Thanks to Bruker scientists Dr. Natalia Erina, Dr. Stefan Kaemmer, and Dr. Chunzeng Li)

Glioblastoma Cancer Cell Cytoskeleton - Peak Force Error (c)

PeakForce QNM investigation of live U-251 Glioblastoma cells. 80x80µm 128x128 peak force error image of cells transfected to overexpress TSF.

Glioblastoma Cancer Cell Cytoskeleton - Young s Modulus (D)

PeakForce QNM investigation of live U-251 Glioblastoma cells. 80x80µm 128x128 Young's modulus image of cells transfected to overexpress TSF.

Multilayered polymer film

PeakForce QNM modulus data of multilayered polymer film overlaid on the surface topography. Scan size 10µm.

Glioblastoma Cancer Cells - Height (A)

PeakForce QNM investigation of live U-251 Glioblastoma cells. 20x20µm 512x512 3D-rendered height image of control cells.

Glioblastoma Cancer Cells - Peak Force Error (B)

PeakForce QNM investigation of live U-251 Glioblastoma cells. 20x20µm 512x512 3D-rendered height and peak force error images respectively of control cells.

Nanopillar Array

Conductivity measurement on nanopillar array using PeakForce TUNA.

P3HT-PCBM Polymer Solar Cell - Adhesion

Dimension Icon 1µm adhesion image of P3HT-PCBM polymer solar cell using pcAFM. (Data courtesy of Noham Sebaihi (CIRMAP – UMONS, Belgium))

P3HT-PCBM Polymer Solar Cell - Photocurrent

Dimension Icon 1µm photocurrent image of P3HT-PCBM polymer solar cell using pcAFM. (Data courtesy of Noham Sebaihi (CIRMAP – UMONS, Belgium))

P3HT-PCBM Polymer Solar Cell - Topography

Dimension Icon 1µm topography image of P3HT-PCBM polymer solar cell using pcAFM. (Data courtesy of Noham Sebaihi (CIRMAP – UMONS, Belgium))

Polymer Brush

MultiMode 8 image of polymer brush structures using ScanAsyst. Scan size 750nm. (Sample courtesy of S. Sheiko, University of North Carolina, Chapel Hill)

Short Circuit of P3HT-PCBM Polymer Solar Cell - Photocurrent

Dimension Icon 10µm photocurrent image of P3HT-PCBM polymer solar cell under short circuit conditions using pcAFM. (Data courtesy of Noham Sebaihi (CIRMAP – UMONS, Belgium)

Short Circuit of P3HT-PCBM Polymer Solar Cell - Topography

Dimension Icon 10µm topography image of P3HT-PCBM polymer solar cell under short circuit conditions using pcAFM. (Data courtesy of Noham Sebaihi (CIRMAP – UMONS, Belgium)

Sn-Pb Alloy Adhesion

Quantitative work function adhesion measurement on a nanostructured Sn-Pb alloy using PeakForce KPFM shows very fine phase structure with strong adhesion differences. 4µm image size.

Sn-Pb Alloy Height

Quantitative work function height measurement on a nanostructured Sn-Pb alloy using PeakForce KPFM. (4µm image size).

Teflon Membrane

30µm scan of a Teflon membrane using PeakForce Tapping.

Sn-Pb Alloy Potential

Quantitative potential measurement on a nanostructured Sn-Pb alloy using PeakForce KPFM shows correct work function differences for the nanoscale domain. 4µm image size.

Elastic image of PHBV in a matrix of polystyrene PS

Elastic The image shown here is of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) in a matrix of polystyrene (PS). Two of the PHBV domains have crystallized and others are supercooled melt. The modulus is painted onto the 3d render of the topographic image to show that the PS is the stiffest (brightest), followed by the crystallized PHBV and finally the liquid PHBV (dark circular areas). Image size 5 microns. Many publications show such use of PeakForce QNM to identify and characterize local phases, see e.g. the study of interphases formed upon annealing by Wang et al. in Macromolecules 2014, 27, 3761 http://pubs.acs.org/doi/abs/10.1021/ma500099b . Quantitative data from PeakForce Tapping has also been used in time-temperature superposition studies (see e.g. Nakajima et al in Microscopy 2014, 63, 193 http://jmicro.oxfordjournals.org/content/63/3/193.long). modulus of polymer blend

Suspended Graphene

Images of suspended graphene membrane acquired in Tapping Mode (grey image, height) and PeakForce Tapping (blue image, adhesion overlaid on height). Image sizes 9 microns. For more details see Clark, N. et al. "Ultrafast quantitative nanomechanical mapping of suspended graphene." Physica Status Solidi (B), 2013, doi:10.1002/pssb.201300137.

Bioscope resolve live cell e-coli

PeakForce Tapping image of live E. Coli cells.

PeakForce QNM ULDPE

Blend of SPP and PEO.

Syndiotactic polypropylene (sPP) and polyethylene oxide (PEO) blend imaged with PeakForce-HR (3 µm at 5 Hz).

PeakForce SECM on nanomesh electrode

(A) 3D topography of a nanomesh electrode (Au-SiO2) covered by EC current skin; (B) line profiles of the topographic and EC variations on the Au-SiO2 surface in (A); and (C) line profiles of the topographic and EC variations from a nanoelectrode array sample. Nanomesh electrode sample courtesy of C. Stelling and M. Retsch, University of Bayreuth. Image courtesy of A. Mark and S. Gödrich, University of Bayreuth. Nanoelectrode array sample courtesy of M. Nellist and Prof. S. Boettcher, University of Oregon.

Elastic modulus of polymer blend image

Image sets (1 and 50) demonstrate a high level of consistency in modulus using FASTForce Volume Nanomechanics mode on a Dimension AFM. The graph of modulus results shows exceptional consistency and repeatability from the Al component. The 12 µm images are generated with an array of 128x128 curves on a 50 nm Al and Cr thin film on Si.

Molecular defect in air

PeakForce QNM images revealing a molecular defect on a polydiacetylene crystal, in air.

Elastic modulus of polymer blend

Syndiotactic polypropylene domains (violet) surrounded by a PMMA (teal) matrix. Topographic data painted with modulus to allow easy identification of components, revealing lamellae formed by syndiotactic polypropylene that propagate through the PMMA matrix. Image size 8 µm.

PeakForce SECM of thiol on Au

PeakForce SECM images of micro-contact printed CH3-thiol self-assembled monolayer (SAM) on an Au substrate: (A) topography variations are <1 nm; (B) PeakForce QNM adhesion force; and (C) electrochemical activity at a lift height of 40 nm. (B) and (C) show quantitatively 700 pN and 108 pA differences in adhesion force and electrochemical current, respectively, between the Au and SAM regions. Image courtesy of A. Mark and S. Gödrich, University of Bayreuth.

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SMIM SRAM multi channel sample images

sMIM images of an SRAM sample. (A) Topography channel,15 μm x 7.5 μm, and ~150 nm tall features; (B) sMIM-C channel as a skin covers on topography; (C) sMIM dC/dV phase channel on topography; (D) sMIM dC/dV amplitude channel on topography. Sample courtesy of Greg Michael Pitner and Professor H. – S. Philip Wong, Stanford University.