Advancing Interactomics Workflows With 4D-Proteomics on the timsTOF Pro

Advancing Interactomics Workflows With 4D-Proteomics on the timsTOF Pro

This webinar took place on May 06th 2020

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Overview

You will hear from leaders in the field using the uncompromised scan speed, sensitivity and robustness of the timsTOF Pro mass spectrometer to elucidate protein-protein interaction networks.

Dr. André Michaelis (Professor Matthias Mann Lab at MPI) will present TIMS and PASEF® technology and its application in successfully performing over 4150 pull-downs and the completion of more than 8300 LCMS measurements in less than 20 weeks for proteome-wide interaction studies in S. cerevisiae.

Professor Peter Jackson (Stanford University) will present on how combining affinity purification mass-spectrometry (AP/MS) and shotgun proteomics with genetic interaction mapping reveals new proteins critical for KRAS-driven cancers.

You can expect to learn

  • TIMS and PASEF provide near 100% duty cycle and 120Hz sequencing speed
  • Robustness and throughput demonstrate the ultimate platform for affinity purification MS workflows
  • How AP/MS using 4D-Protemics workflows uncover new proteins critical in KRAS cancer
  • 8300 LCMS runs are accomplished in 20 weeks' time, averaging 415 LCMS runs per week (or nearly 60 runs per day)

Speakers

André Michaelis
André Michaelis
PhD researcher, Matthias Mann lab Proteomics and Signal Transduction, Max Planck Institute for Biochemistry
Dr. Peter Jackson
Dr. Peter Jackson
Baxter Laboratory for Stem Cell Biology Department of Microbiology & Immunology at the Stanford University School of Medicine