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Robust and accurate gradient delivery with intelligent diagnostics

Trouble-free OMICS knowledge generation

The nanoElute system provides excellent chromatographic performance even for very short gradients in both separation modes. With a trap column installed, the focus is on speed and robustness, however the chromatographic performance is maintained due to new nano-columns and unique back-flushable trap cartridge.

With trap column

Without a trap column installed, ultimate separation power is delivered where the trap column is elegantly by-passed by virtue of the novel valve geometry.

Without column trap

The unique instrument configuration allows the user to analyze samples in both “with trap” and “without trap” mode in the same sample batch without physically changing the hardware setup.

Separation of complex samples with a long column and gradient has been demonstrated both with and without trap column. The retention time and area reproducibility is excellent displaying suitability for all proteomics applications, including the most demanding ones.

Method

Predigested Bovine Serum Albumin (Bruker, Billerica, MA) was used as a representative for a simple proteomics sample (25 fmol/µl).
HeLa cell lysate was prepared according to Beck et al.¹ and acidified with formic acid to a final concentration of 200ng/µl. For the simple sample, the tryptic peptides were separated on a novel nano-UHPLC system using a 10min reversed phase gradient (2% - 35% solvent B in 10min. - 0.1% FA in ACN), on standardized 15cm 75µm bore column with C18 resin. The HeLa sample was separated with a reversed phase gradient (from 2% -24% in 90min, 24-48 in 10min. solvent B - 0.1% FA in ACN), on a standardized 40cm 75µm bore columns with C18 resin. Data was acquired with an impact II QTOF MS (Bruker Daltonik, Bremen, Germany) equipped with a CaptiveSpray ion source.

References:
[1] Beck, S., Michalski, A., Raether, O, Lubeck, M., Kaspar, S., Goedecke, N., Baessmann, C., Hornburg, D, Meier, F., Paron, I., Kulak, N.A., Cox, J. and Mann, M. “The Impact II, a Very High-Resolution Quadrupole Time-of-Flight Instrument (QTOF) for Deep Shotgun Proteomics.” Mol Cell Proteomics. 2015 Jul;14(7):2014-29.

Direct infusion for fast analysis

Direct Infusion for fast analysis

The unique valve design also allows ”Direct Infusion” for fast runs of simple samples e.g. BSA at 2 μl/min.

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.