Brochure: rapifleX, 07-2019
The rapifleX is the most advanced MALDI-TOF/TOF system on the market today and it is also the most adaptable. Whether you are looking at top-down sequencing for Biotherapeutics and Biosimilars, glycan structure analysis and confirmation, and localization of disulfide/ trisulfide bond determination and scrambling analysis, the rapifleX is the best instrument...
The rapifleX is the most advanced MALDI-TOF/TOF system on the market today and it is also the most adaptable. Whether you are looking at top-down sequencing for Biotherapeutics and Biosimilars, glycan structure analysis and confirmation, and localization of disulfide/ trisulfide bond determination and scrambling analysis, the rapifleX is the best instrument for the job. The system combines state-of-the-art technology with advanced informatics into a system that gives you speed, robustness, versatility and confidence.
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Application Note: MALDI-TOF Mass Spectrometry Imaging (MSI) for Distribution Analysis of Anthocyanins in Strawberry Fruits, 03-2019
Imaging mass spectrometry (MSI) via matrix-assisted laser desorption / ionization
(MALDI) TOF MS enables the simultaneous detection and localization of molecular
species across a broad molecular mass range. In biological samples, diverse classes
of biomolecules may be sought, including metabolites, sugars, lipids, peptides,
and proteins.
Imaging mass spectrometry (MSI) via matrix-assisted laser desorption / ionization
(MALDI) TOF MS enables the simultaneous detection and localization of molecular
species across a broad molecular mass range. In biological samples, diverse classes
of biomolecules may be sought, including metabolites, sugars, lipids, peptides,
and proteins.
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Poster: High-throughput screening of Deubiquitylase enzyme (2088), 02-2016
Ubiquitylation regulates a large number of biological processes in eukaryotic cells and it is reversible by specific cleavage through deubiquitylases (DUBs). DUBs are key regulators of the ubiquitin system and play an essential role in cell homeostasis1. As DUBs are dysregulated in various diseases, they are considered attractive drug Targets for cancer...
Ubiquitylation regulates a large number of biological processes in eukaryotic cells and it is reversible by specific cleavage through deubiquitylases (DUBs). DUBs are key regulators of the ubiquitin system and play an essential role in cell homeostasis1. As DUBs are dysregulated in various diseases, they are considered attractive drug Targets for cancer treatment. High-throughput screening (HTS) methods using physiological substrates is important for the identification of novel drug candidates. Several chemical probes2 as well as fusions of fluorogenic reporters3,4 to the C terminal glycine of ubiquitin are widely deployed. However, these methods make use of artificial and non-physiological substrates, thus are not suitable for assessing the linkage specificity of DUBs. As these are artificial substrates do not contain hysiological isopeptide bonds, screening assays using these substrates could potentially identify compounds that might not inhibit the deubiquitylation of physiological substrates. It is possible to undertake DUB assays with more physiologically related diubiquitin molecules, however these assays use low throughput SDS–PAGE methods and require large amounts of enzymes and substrates.
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