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FT/CW System

The basis for multifrequency and multiresonance FT-EPR

The E 580 is a highly versatile instrument that allows all pulse experiments of today and is prepared for the challenges of tomorrow.

PatternJet-II Channel
PatternJet-II Channel

The next generation, SpecJet-II and PatternJet-II, is now on the market to further enhance speed and precision of Pulse-EPR data acquisition. These new developments, combined with the flexibility, sensitivity and reliability of the FT/CW-EPR bridges and the Flexline resonators, make the ELEXSYS E580 an unsurpassed research instrument for the investigation of paramagnetic species in all disciplines of science.

  • FT-EPR and Electron Spin Echo techniques as the basic detectors for the EPR spectrum and electron spin relaxation times
  • ESEEM and 2D-HYSCORE to measure spin density distribution and distances through the hyperfine interaction between electron and nuclear spins
  • SECSY and EXSY to measure correlations and exchange rates
  • Pulse-ELDOR and DEER to measure long range distances by electron-electron spin dipolar coupling
  • Pulse-ENDOR to measure hyperfine interactions and nuclear spin relaxation
  • Laser triggered experiments to investigate chemical reactions and triplet states

Background

In the last decade Pulse-EPR matured to a well established tool for the investigation of paramagnetic species. From the very beginning Bruker set the milestones in commercial Pulse-EPR instrumentation with the ESP 380 introduced in 1987.  The next landmark was set by the successor model ELEXSYS E 580 and the new high speed digital devices PatternJet and SpecJet. For the first time in history it was possible to average FIDs and echoes at a rate governed by spin relaxation and not hampered by hardware constraints.

Accessories

Another cornerstone of the spectrometer, the probehead, has been in the focus of development over many years. The result is the legendary Flexline probehead series.
 
The ELEXSYS E 580 FT/CW X-Band spectrometer is also the platform for multi-frequency extensions. Based on the X-Band spectrometer, frequency conversions are available to W-Band (E 680), Q-Band (SuperQ-FT), S-Band, (SuperS-FT) and L-Band (SuperL-FT). The unique design concept allows to transform all features of the X-Band to the additional frequency in a dual-band spectrometer.

Xepr for experiment design and data handling

Unprecedented flexibility and ease of use are the attributes of the Xepr software. Whether you are dealing with a simple echo decay or a complicated multiple-pulse 2D experiment, the graphical user interface of Xepr ensures easy instrument control, experiment definition and execution. Numerous novel processing tools, display capabilities and data representations open up new dimensions in data analysis as well.

Xepr software

PatternJet-II Channel
PatternJet-II Channel

SECSY and EXSY

Spin Echo Correlation Spectroscopy (SECSY) and Exchange Spectroscopy (EXSY) are 2-dimensional techniques. The positions of cross peaks in the 2D spectrum are used to correlate spectral peaks with each other and the cross peak amplitudes carry the information about exchange rates. These experiments are standard features of the E 580 spectrometer and are supported by predefined PulseSPEL programs.

EXSY spectrum
Spin Echo Correlation Spectroscopy (SECSY) and Exchange Spectroscopy (EXSY) are 2-dimensional techniques.

Laser Triggered Experiments

An external event, e.g. a laser flash, can be used to generate a paramagnetic state. For this type of experiment the instrument can be triggered externally or alternatively the instrument can send a trigger pulse to activate an external device. Precise synchronization between the external event, the microwave pulses and the data acquisition is established. Any pulse-EPR experiment can be run in combination with the external trigger function.

ESE detected field sweep spectrum
An external event, e.g. a laser flash, can be used to generate a paramagnetic state.

Pulse ELDOR and DEER

Electron-Electron Double Resonance (ELDOR) and Double Electron-Electron Resonance (DEER) are acronyms for the same technique. With this technique the dipolar interaction between electron spins is measured. This information directly translates into the distance between the electron spins. Distance ranges of 10 to 80 Angstrom are covered. In recent years the combination of site directed spin labeling and DEER became one of the most popular tools in structural protein research.

DEER time domain signal
With this technique the dipolar interaction between electron spins is measured.

ESEEM and 2D-HYSCORE

Electron Spin Echo Envelope Modulation (ESEEM) is one of the most popular techniques to measure the hyperfine interaction (HFI) parameters between electron spins and nearby nuclear spins. From the analysis of the ESEEM signals detailed information about electron spin density distribution, distances and bonding angles is gained. 2D-HYSCORE is a technique also based on the ESEEM effect and is the method of choice for frozen solutions and powders. All ESEEM-type of experiments are standard features of the E 580 and are supported by dedicated acquisition and processing tools in Xepr.

2D-HYSCORE spectrum
Electron Spin Echo Envelope Modulation (ESEEM) is one of the most popular techniques to measure the hyperfine interaction (HFI) parameters between electron spins and nearby nuclear spins.

LabScape

Service and Life Cycle Support

Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.

LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory

Download LabScape Brochure