Walking Along Chromosomes: Visualizing in situ Chromosomal Structure with Single Molecule Localization Microscopy
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Understanding chromosomal structure in situ is critical to understanding nuclear function. This webinar shows how OligoSTORM and single molecule localization microscopy can be used to visualize in situ chromosome structure. By incorporating microfluidics and sequential labelling strategies can be employed to “walk” along chromosomes to visualize chromosome regions that form distinct structures in the form of compartments. Date presented, up to 8 megabases of human chromosome 19, will show that in addition to regional compartments, there are suggestions that there may be differential organization of chromosomes in maternal and paternal homologues.
The functional implications of these findings and the types of data that can be generated with this technique in terms of understanding chromosomal function are also discussed.
- Single molecule localization microscopy and OligoSTORM for imaging chromosome
- Sequential fluorescent labelling to image complex molecules
- Interpreting 3-D images of chromosomal structure
- Functional implications of 3-D structure of chromosomes