Metabolomics solution web

Simplified T-ReX LC-QTOF metabolomics solution

Because every step matters…

Several crucial steps are important for confidently identifying compounds relevant for dynamic metabolic processes; including sample preparation, data acquisition and data evaluation.
Meaningful insights in a biological context can only be obtained if all of these workflow steps are controlled. To simplify and harmonize these, Bruker, in collaboration with Prof. Liang Li and his team at the University of Alberta, Canada, have developed the T-ReX LC-QTOF solution.

…the “T-ReX LC-QTOF solution” includes everything to provide researchers with a head start in non-targeted metabolomics:

  • The first critical step in non-targeted metabolomics is a reproducible sample preparation. Hence, Standard Operation Procedures (SOPs) for preparing typical clinical research samples, including urine and plasma, are provided as part of this metabolomics solution.
  • Acquisition of high quality LC-MS/MS data follows sample preparation in non-targeted metabolomics workflows, with the T-ReX LC-QTOF solution, no LC-MS/MS parameter optimization is required. To analyze large sample cohorts which require high retention time stability the ELUTE UHPLC  in combination with the dedicated T-Rex Elute Metabolomics-kit: RP is provided. The Reversed-Phase LC column kit enables matching of retention times to values in the Bruker HMDB Metabolite Library 2.0. The impact II for MS/MS data acquisition uses optimized parameters and its robust performance is the basis for high quality data acquisition, enabling extensive profiling studies of complex samples.

Following data acquisition, the MetaboScape software provides streamlined data evaluation, enabling automatic and confident identification of relevant known compounds:

  • Unique T-ReX 3D feature extraction technology is the key to subsequent metabolite identification and statistical data evaluation. By automatically conducting parameter free retention time alignment and region complete extraction, all relevant information is extracted to enable confident metabolite identification and at the same time false negative rate in statistical analysis is reduced.
  • The Bruker HMDB Metabolite Library 2.0 as part of this metabolomics solution provides MS/MS spectra for > 800 metabolites with retention time information for >600 relevant metabolites. The library is a prerequisite for confident identification of relevant markers.
  • In-silico generated MS/MS spectra in the Bruker MetaboBASE Personal Library 2.0  enables tentative identification of relevant targets which are not yet available as pure reference standards.
  • Annotation Quality (AQ) scoring provides a fast overview giving confidence in each annotation parameter.
  • Pathway mapping functionality enables the setting of identified compounds into a biological context.
Metabolomics solution
T-ReX LC-QTOF solution – Simplifies non-targeted metabolomics by supporting all crucial workflow steps
Prof. Liang Li

“We are delighted to have collaborated with Bruker to produce the comprehensive T-ReX LC-QTOF solution. The complete out of the box solution provides the basis for high confidence identification of relevant known endogenous metabolites and enables to set these into a biological context using pathway mapping. This solution will provide researchers a head start in non-targeted metabolomics for typical research samples like urine or plasma.”

Dr. Liang Li, Professor of Chemistry, University of Alberta, Canada

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.