QCI-P-CRP_Banner.jpg

Versatile Quadruple Resonance CryoProbe for Structural Biology Applications

New, versatile proton-optimized quadruple resonance NMR ‘inverse’ probe

Applications

The new QCI CryoProbe™ - a proton-optimized quadruple resonance NMR ‘inverse’ probe - increases sensitivity by about 400% on 1H and 13C compared to a room temperature QXI probe. 

The versatile probe features four fully independent channels that enable simultaneous decoupling on multiple nuclei such as 13C, 31P and 15N. This cost-efficient adaptability eliminates the need for probe changes, making it ideally suited to the study of RNA or DNA, whilst also enabling research on proteins, small molecules and metabolomic samples, something which has made Bruker’s TCI CryoProbe™ a success.

Several recent discoveries in RNA research make this probe a very timely introduction to the field of cutting edge NMR research. The cryogenically cooled preamplifier for deuterium guarantees highest sensitivity for the lock channel, resulting in excellent stability of the spectrometer. This also permits the use of as little as a few percent of deuterated solvents.  In addition the QCI CryoProbe™ is equipped  with cold preamplifiers for 1H  and 13C. The probe is compatible with shaped tubes for optimum performance on lossy samples.

  • Proton-optimized quadruple resonance NMR probe for 5mm samples
  • Sensitivity increase by a factor of four on 1H and 13C 
  • Four fully independent channels (plus lock channel) 
  • Simultaneous decoupling on multiple X-nuclei such as 13C, 31P and 15
  • Cooled 2H preamplifier for excellent stability 
  • Prepared for shaped tube application
  • Fitted with an actively shielded single axis Z-gradient for 5 mm sample diameters
  • Standard sample temperature range -40°C – 150°C
  • ATM and CryoFit compatible 
  • Cost-efficient adaptability eliminates the need to change probes 
  • Ideally suited for the study of RNA or DNA 
  • Enables research on proteins, small molecules and metabolomic samples
  • Optimized for 1H, 31P and 13C detection

Unbuffered Male Urine Sample

  • Sample: 350 µl unbuffered male urine in shaped sample tube
  • Top: Presat ns = 8
  • Left: HSQC aromatic region
  • Right: 2D J-RES aliphatic region
qci_urinespec.jpg
Unbuffered Male Urine Sample

2D 13C observe TOCSY

2D 13C observe TOCSY with 31P & 15N & 1H decoupling, 4 mg 15N/13C labeled RNA 14-mer, experiment time 40 minutes


QCI-2Dspec.jpg
2D 13C observe TOCSY

Protonless HiRes Bio-NMR

Protonless HiRes Bio-NMR: 3D CBCACO-IPAP of 1mM Ubiquitin 15N/13C labeled, taken at 600 MHz (ns=2,TD=1024x64x256, experiment time 10 hours)

qciprotonlessspec.jpg
Protonless HiRes Bio-NMR

1H-15N NN-COSY

1H-15N NN-COSY (H Bond correlation) of 4 mg (~2mM) 15N/13C labeled RNA 14-mer, taken at 600 MHz (ns=8, td= 2kx128, experiment time 25 min, te= 283K).

*RNA standards by Silantes GmbH

qci_NN-COSY.png
1H-15N NN-COSY

LabScape

Service & Life Cycle Support for Magnetic Resonance and Preclinical Imaging

Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.

LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory

Download LabScape Brochure