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Fluorescence Optical Microscopy Webinars

An Open and Free Resource for Fluorescence Microscopy Owners and Users in Life Sciences

Our fluorescence microscopy webinars are available on our YouTube channel — www.youtube.com/BrukerNanoSurfaces. The links below will take you to the respective recording online. No registration is needed to watch a prior webinar. Sign up for email notifications about Bruker's upcoming webinars.

Recent Webinar

June 7, 2017

Quantitative Single Molecule Localization Microscopy
Single molecule localization microscopy allows for the direct investigation of the molecular positions and distribution of proteins within the cellular environment, creating data sets that can be analyzed conveniently through numerous statistical analysis methods. The statistical tools available within the Bruker Vutara SRX software suite will be investigated in this webinar.

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Past Webinars

Webinar Content
March 2017

In Vivo Visualization of Stromal Macrophages via Label-free FLIM-based Metabolite Imaging
with guest speaker Joseph Szulczewski from University of Wisconsin
Listen to this webinar discussing University of Wisconsin research where they use a novel strategy that non-invasively identifies tumor-associated macrophages in the intact mammary tumor microenvironment.

ABSTRACT
Macrophage infiltration and recruitment in breast tumors has been correlated with poor prognosis in breast cancer patients and has been linked to tumor cell dissemination. Much of our understanding comes from animal models in which macrophages are labeled by expression of an extrinsic fluorophore. However, conventional extrinsic fluorescence labeling approaches are not readily applied to human tissue and clinical use.
We report a novel strategy that exploits endogenous fluorescence from the metabolic co-factors NADH and FAD with quantitation from Fluorescence Lifetime Imaging Microscopy (FLIM) as a means to non-invasively identify tumor-associated macrophages in the intact mammary tumor microenvironment. Macrophages were FADHI and demonstrated a glycolytic-like NADH-FLIM signature that was readily separated from the intrinsic fluorescence signature of tumor cells. This non-invasive quantitative technique provides a unique ability to discern specific cell types based upon their metabolic signatures without the use of exogenous fluorescent labels. Not only does this provide high resolution temporal and spatial views of macrophages in live animal breast cancer models, this approach can be extended to other animal disease models where macrophages are implicated and has potential for clinical applications.

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May 2016

Super Resolution – Confocal Correlative Imaging
Super Resolution imaging using single molecule localization techniques (PALM, STORM) provides the highest resolution available for a fluorescence microscopy modality. While super resolution provides an opportunity to examine the relationship of proteins and structures down to 20 nm, interpreting the data within a larger context can sometimes be difficult.

Acquiring confocal images as references along with super resolution images provides context for visual interpretation as well as providing a guide for analysis of super resolution data. The Vutara 352 with Opterra SR option provides a platform for correlative super resolution confocal imaging. The Opterra SR high speed confocal can be used for sample navigation as well as acquiring references images, which can be merged to allow interpretation and guided analysis of super resolution data.
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June 2015

Light as the Independent Variable: Photomanipulation Techniques for Confocal and Multiphoton Microscop
A variety of techniques are available to biological microscopists that allow them to use light as a stimulus to produce measurable changes in their sample of interest. These techniques include uncaging, fluorescence recovery after photobleaching (FRAP), photoactivation, photoconversion and photoablation.

We will cover the basic principles of these various techniques and examine their value in various experimental protocols. We’ll show examples of the various techniques using data collected on Bruker Ultima multiphoton microscopes and Opterra multipoint scanning confocal microscopes.
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April 2015

Quantitative Super-Resolution Applications in Life Science Using Single Molecule Localization Microscopy
In this webinar we will discuss examples of how researchers have used this quantification capability of SML to address key biological questions. The Vutara 350 super-resolution microscope has thus been engineered to enable researchers to fully harness the power of quantitative SML imaging by offering, three-dimensional, multi-color, live cell imaging capability coupled with custom-designed Vutara SRX software.
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March 2015

Exploring in vivo Neural Circuit Activity with Simultaneous All Optical Manipulation and Recording
with guest speaker Dr. Adam Packer
Dr. Packer will review his recent work in which he utilized a Spatial Light Modulator (SLM) interfaced to a multiphoton microscopy system in order to simultaneously use optical stimulation and optical recording to study neural networks. A major benefit of the use of the SLM is that tens of neurons can be simultaneously stimulated, while at the same time imaging neuronal activity in a field using a calcium indicator.
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February 2015

Super Resolution Imaging – How Fast Can You Go?
We will review general principles of single molecule localization super resolution techniques, and the instrumentation requirements for a system capable of acquiring images at rates that are compatible with live cell experiments. We will also review experimental protocols required for successful live cell super resolution experiments, including instrument settings and sample preparation.
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January 2015

Multiphoton Imaging of Neuronal Activity in vivo - Mapping Neuronal Activity During Sensory Stimulation and Conditioning Trials in Awake Mammals
Learn how researchers image neurons firing during behavioral tasks in order to understand how they function as groups and how that pattern of activity changes as an animal progresses through a conditioning trial series.
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December 2014

Live Cell Super-Resolution Imaging
In this webinar we will cover the technology and sample preparation optimization techniques necessary for successful live single cell molecule localization (SML) super-resolution imaging. Speed of imaging and data processing, laser intensity, and availability of live cell compatible fluorescent labels are all key factors that need to be considered when designing a live cell super-resolution experiment. The Bruker Vutara 350 super-resolution microscope has been engineered to be live cell ready; the sCMOS camera enables video-rate imaging, parallel processing computer architecture and multi-emitter fitting enable real time localization, and high laser powers enable rapid photoswitching of fluorophores. Combining Vutara 350 live cell ready technology with the rapidly evolving field of fluorophore engineering for live cell applications promises to overcome the limitations faced thus far in live cell super-resolution imaging.
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October 2014

Multidimensional Imaging for Complete Investigation of Cellular Events
In this webinar the technical differences between Opterra and point or disc scanners will be presented, as well as live-cell application data demonstrating some of the possibilities this system can bring to your research.
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October 2014

Harnessing the Power of Super-Resolution Microscopy
In this webinar we will focus on the principles of SML super-resolution microsocopy and how to optimize sample preparation for SML imaging. We will discuss the details of dye selection, sample labeling, and imaging protocols. We will also present examples of data and images that can be obtained through careful sample optimization in cell culture and tissue specimens.
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September 2014

Multiphoton Fluorescence Lifetime Imaging (FLIM)
Evaluating Tumor Development and Efficacy of Anticancer Pharmacologics
In this webinar we will cover basic technical principles of multiphoton FLIM, as well as present data showing the utility of multiphoton FLIM as a tool for studying tumor development as well as a predictive assay for evaluating efficacy of anticancer pharmacologic agents.
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August 2014

Super-resolution Microscopy and Its Applications in Fast and Complex Biological Systems
Super-resolution microscopy has revolutionized the field of biological imaging by providing new insights into biological processes in fields as diverse as developmental biology, neuroscience, cardiovascular research, genetics, infectious disease, and DNA/chromatin structure. The Vutara 350 super-resolution microscope offers a ten-fold improvement in resolution in comparison to traditional light microscopy techniques and is capable of achieving resolutions of 20 nm laterally and 50 nm axially. The Vutara 350 is based on a patented 3D biplane single molecule localization platform.

In this webinar we discuss the basic principles of operation and features of the Vutara 350 super-resolution microscope. The capability to do 3D multicolor imaging, high speed live cell imaging, 3D particle tracking, and z-stacking in various biological systems such as cells, tissue, drosophila, C. elegans, bacteria and virus makes the Vutara 350 very versatile.
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June 2014

A Deeper Understanding of In Vivo Immune Cell Function Through the Use of Multiphoton Intravital Microscopy
Multiphoton Intravital Microscopy (MP-IVM) is an advanced imaging technique that allows visualization of fluorescently labelled cells in their natural environment, allowing imaging at depths of hundreds of microns into intact organs in vivo.

MP-IVM has proven to be a revolutionary tool in the study of immune cell function, allowing observation of the functions in lymphnoid as well as non-lymphoid tissues, such as sites of infection or tumors. In addition to qualitative observation of immune cell behavior, MP-IVM lends itself to quantitative measurements of cell migration, cell-cell interactions and the activity of signaling pathways.
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March 2014

The Unique Scanning Mechanism Design of the Opterra Multipoint Scanning Confocal Microscope
This webinar will discuss the unique scanning mechanism design of the Opterra™ Multipoint Scanning Confocal Microscope, which lends itself to acquisition of spectral data sets at speeds appropriate for imaging of live cells and small organisms. Opterra's 1-D array of 32 pinholes, coupled with an Amici prism, allows collection of full spectral data sets of 15 channels at speeds up to 4 spectral data sets per second. Spectral data sets can be acquired with time lapse, Z series and stage control, thus allowing a full range of multidimensional data acquisition methods, including time-based volumetric imaging, 3-D stage montages and timed-based acquisition from multiple stage locations.
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