What you will discover

Cardiac morphology and function assessment of mice by magnetic resonance imaging is of increasing interest for a variety of mouse models in pre-clinical cardiac research, such as myocardial infarction models or myocardial injury/remodeling in genetically or pharmacologically induced hypertension. With standard room temperature coils, signal-to-noise ratio (SNR) constraints limit image quality and blood myocardium delineation, which crucially depend on high spatial resolution. The markedly improved image quality with the use of the MRI CryoProbe – by better delineation of myocardial borders and enhanced depiction of papillary muscles and trabeculae – facilitates more accurate cardiac chamber quantification, due to reduced intra-observer variability (1).

1. Wagenhaus B, Pohlmann A, Dieringer MA, Els A, Waiczies H, Waiczies S, Schulz-Menger J, Niendorf T. Functional and morphological cardiac magnetic resonance imaging of mice using a cryogenic quadrature radiofrequency coil. PLoS One 2012;7(8):e42383.
2. Waiczies H, Guenther M, Skodowski J, Lepore S, Pohlmann A, Niendorf T, Waiczies S. Monitoring dendritic cell migration using 19F / 1H magnetic resonance imaging. J Vis Exp 2013(73):e50251.

A comprehensive view of brain inflammation during the pathogenesis of autoimmune encephalomyelitis (EAE) can be achieved with the aid of high resolution non-invasive imaging techniques. With the MRI CryoProbe, inflammatory infiltrates within various regions of the brain, can be studied in detail – even without the use of contrast agents – and show excellent correspondence with conventional histology. This opens up the real opportunity to follow neuroinflammatory processes even during the early stages of disease progression. High-resolution MRI does not only complement conventional histology but also enables longitudinal studies on the kinetics and dynamics of immune cell infiltration (2-4).

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3. Lepore S, Waiczies H, Hentschel J, Ji Y, Skodowski J, Pohlmann A, Millward JM, Paul F, Wuerfel J, Niendorf T, Waiczies S. Enlargement of cerebral ventricles as an early indicator of encephalomyelitis. PLoS One 2013;8(8):e72841.
4. Waiczies H, Millward JM, Lepore S, Infante-Duarte C, Pohlmann A, Niendorf T, Waiczies S. Identification of cellular infiltrates during early stages of brain inflammation with magnetic resonance microscopy. PLoS One 2012;7(3):e32796.

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