SCAN HEAD |
|
| Scanning Method |
Matched pair of 6 mm Cambridge galvanometers with raster and spiral scanning capabilities |
| Field of View |
~1.404 mm x 1.404 mm with 16x objective (up to 28.3 mm FN) |
| Scan Speed |
Raster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64
Spiral scan: 6 fps at 512 x 512, ~30 fps at 64 x 64 |
| Scan Customization |
User-definable straight, freehand and circular (infinite) linescan with included software; user-definable pixels per line and lines per scan from 1 - 2048; up to 128x scan zoom; 360° of scan rotation; point scan |
| Uncaging Option |
Second set of matched 3 mm or 6 mm Cambridge galvanometers in the same scan head to provide high-precision visible or multiphoton laser sample photomanipulation |
| High-Speed Imaging Option |
8 kHz resonant galvanometer; 30 fps at 512 x 512, >1300 fps at 512 x 8 region of interest |
DETECTORS |
|
| Reflected Non-Descanned |
1 - 4 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs |
| Transmitted Non-Descanned |
1 - 2 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs |
| Dodt |
Single Hamamatsu PMT for DIC-like image collection |
| Transmitted |
Single Hamamatsu PMT for transmitted light image collection |
| Camera |
Standard C-mount camera port built into scan head |
OPTICAL INPUTS |
|
| Multiphoton Laser |
Optimized for multiphoton laser input from 690 - 1700 nm |
| Epifluorescence |
High-Powered LED in epifluorescence turret |
| Visible Laser |
Fibered laser inputs for visible laser introduction |
| Ultima Laser Rating |
Class 1 (contains Class 3b & Class 4 lasers) with light box |
| Helios Laser Rating |
Class 3b |
| LED |
Full-field photoactivation with LED module |
MOTOR CONTROL |
|
| Bruker X,Y Stage |
Variable height with ~15 cm X and ~7.5 cm Y movement and ~0.3 μm step size |
| Bruker X,Y Microscope Base |
Fine and coarse-movement platform for scope with ~35 mm travel and 0.1 μm step size |
| Bruker Z-Focus |
Range of 30 mm with ~0.2 μm step size |
| Bruker Z-Piezo |
Travel range of up to 1000 μm with 0.05 μm step size |
| Bruker Orbital Nosepiece |
Motorized control of objective angle and rotation |
SOFTWARE |
|
| Prairie View Imaging |
Software fully integrated with scan head for easy imaging; customizable scan settings for optimization of specimen excitation; integrated control of laser power and PMT high voltages |
| Z-Series |
Easy creation of depth stacks with user-customizable slice number, step size and laser power |
| T-Series |
Easy creation of complex series involving Z-Series and triggered images |
| Stage Montage |
Atlas Imaging simplifies setup and optimizes acquisition of 2D and 3D stage montages |
| Peripherals Integration |
Wavelength and power control available for multiphoton and visible laser launches |
| Photoactivation |
User-defined points and regions with synchronized laser modulation |
| Regions of Interest |
User-defined regions for faster scanning capabilities |
| Brightness Over Time |
Intensity mapping and plotting for user-defined regions over time |
| Voltage Inputs/Outputs |
Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with external devices |
