To provide a short update on our customers and solutions in front line response to COVID-19 NMR research.
A short update on COVID-19 NMR project and how NMR can applied to addressed the secondary structure of SARS-CoV-2 RNA, and determined the protein stuctures including non-structural proteins and nucleocapsid of SARS-CoV-2 virus. An update on molecular phenotyping of SARS-CoV2 patients using NMR are disscussed. The initial result indicated that there is a high promise of using this approach for developing novel SARS-CoV 2 diagnostic test based on concept called Phenoconversion.
Researchers from clinical and biomedical fields.
Provide brief introduction of NMR on Metabolomics Application and discussion on how to have a good start.
Applications of Metabolomics approach are very diverse, namely Natural Products Characterization, Prediction of phytomedicine efficacy and in vivo pharmacokinetic study, Quality control of herbal medicines and material authenticity, drug treatment in clinical study (pharmacometabolomics), impact of nutrition on health (foodomics) and many more. The reasons on why NMR is commonly used for metabolomics analysis are discussed. Appropriate objective/experimental design in Metabolomics research is utmost important and could be the determining factor on the success of the study. Factors that influences on chemical shifts are also discussed.
Researchers from variety fields who want to explore on NMR-based Metabolomics approach.
Provide brief introduction of NMR spectrometer.
Brief tour to new corporate Bruker Website 2021 and how to get the Free TopSpin Academia License. Introduction to NMR Spectrometer namely Benchtop and High Field instruments. Key components of NMR Spectrometer including Magnets, Electronic Console, Probes are discussed. The importance of Lock and Shimming as mandatory steps in NMR acquisition are explained.
For newcomers to NMR Spectroscopy and undergraduates
Metabolic phenotyping using nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS) has been applied to the characterization of the metabolic effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on human blood plasma. The hybrid NMR-MS model demonstrated a sensitivity of 100 % for SARS-CoV-2 infection, showing it to be an exceptionally powerful tool for the metabolic discrimination of samples infected by the SARS-CoV-2 virus. The signature of SARS-CoV-2 positivity includes elements of liver dysfunction, dyslipidaemia, diabetes, and coronary heart disease risk. Heating of blood samples to deactivate SARS-CoV-2 virus can lead to incorrect biomarker identification. Heat-treatment (56 °C for 30 minutes; the standard protocol used for inactivation of SARS CoV-2 in blood samples analyzed in laboratories without high-level biosecurity facilities) shown to degrade lipoproteins and differentially alter metabolic information. Data related to SARS CoV-2 obtained from heat-treated samples are largely uninterpretable and are of limited research value.
Researchers who are working on clinical field, typically COVID-19 disease.
Good selection of NMR experiment could ensure optimize results which in turn deliver the maximum information of the mixture.
Researchers who plan to collect good NMR data at the highest efficiency.
NMR Spectroscopy play a vital role in development of herbal medicines. “Classical” medicinal plant research required several fractionation steps until the isolation of pure chemical constituents. NMR spectroscopy is then applied to identify and characterize the pure natural products. As the technologies developed, the trends in herbal medicines research have changed. Nowadays, analyzing mixture sample from herbal medicines become routine especially the integration of chemometrics approach to NMR spectroscopy, or also known as Metabolomics. Therefore, NMR-based metabolomics could be applied to authenticate the nutraceutical products and medicinal materials. Particularly, in addition to taxonomic/variety information related to the herbal species, this method is powerful to discriminate the geographical and seasonal differences of herbal materials. Hence, making quality control of phytomedicines possible when non-targeted analysis (Multivariate statistical analysis) is applied on NMR data.
Researchers from National center of natural products, Department of Pharmacognocy, Center of Phytochemistry, Natural Products Laboratory, Institue of Drug and Food Control
Topics: Introduction to NMR spectroscopy
Layman and undergraduates
NMR Application Overview
Layman and graduates