Array of eight channels/three spot enables 8800 interactions in 24 hours
Continuous Flow SPR Analysis
The SPR-24 Pro system is a continuous flow biosensor, delivering sample and reagents to the detection sensors as continuously flowing streams. When sample or reagent injection stops, a “continuous flow buffer” is passed over the sensors.
The formulation of the continuous flow buffer in SPR assays can have a significant impact on the molecular interactions being measured. In many applications, measuring target interactions in a variety of buffer environments is required.
The Frame Inject feature in the Sierra SPR-24 Pro system allows the investigation of condition dependent binding / mode of action studies. The feature requires just a fraction of each buffer condition that replaces the system buffer while usage. The pre-association, as well as the dissociation occurs under the conditions, that should be investigated. After dissociation end, frame solution buffer is replaced and system buffer is flowing through the microfluidic system again. Thus experimental time and reagent costs are kept low. The same buffer can be directed to all flow cells, or up to eight different buffers can be assayed simultaneously.
Individual Needle Control
The individual needle control (INC) feature in the Sierra SPR-24 Pro system allows the eight sample pickup needles to be operated independently as well as in unison. Researchers have the flexibility to choose any combination of 1 – 8 needles to design their optimal assay.
Individual needle control maximizes the performance, flexibility, and scope of applications with the Sierra SPR-24 Pro, while also saving time, materials, and resources when throughput is not the main requirement.
The eight-needle probe head can simultaneously process 8 sample or reagent solutions. Using the INC feature, users can choose to use any number and/or combination of needles at any time during analysis.
The Sierra SPR-24 Pro delivers faster throughput across a wide range of applications, from kinetic assays with a different target on each sensor to detailed analysis employing multiple in-line controls.
The system uses Bruker’s Hydrodynamic Isolation (HI) continuous flow microfluidic technology to address samples as flowing streams onto the 24 spot SPR+ sensor array. When integrated with an optional plate handling robot, the Sierra SPR-24 Pro instrument can assay more than 4400 samples per day, generating more than 8800 control subtracted binding responses.
Sierra SPR-24 Pro can be easily integrated into an automated scheduling software via API.
The flexible Sierra SPR-24 Pro system is ideal for high throughput applications. It offers 24 detection spots, which are addressed by 8 injection needles. Individual needle control (INC) gives researchers the option to choose any combination of 1 to 8 needles, to optimize the performance, flexibility and scope of applications.
The 24-sensor detection array is laid out in 8 flow cells, each containing 3 sensors. Up to 8 samples can be analyzed simultaneously, generating up to 24 binding measurements per analysis cycle. All 24 sensors can also be addressed fully independent of the others, providing maximum assay design flexibility.
The versatile system can also save time and materials in the lab, particularly if throughput is not the main requirement.
Up to eight buffers can be used simultaneously in the instrument, using the frame inject feature. The ability to evaluate different pHs, ionic strength, detergent or solvent concentration, as well as the effect of co-factors or inhibitors, in a single assay results in simpler, optimized assays that ultimately increase throughput and productivity. The four-sensor per channel design provides maximum flexibility for control analysis, while maintaining throughput for the most complex analyses.
The design of Sierra SPR-24 Pro maximizes control analysis flexibility, enabling high throughput for the most complex analyses. Samples can be simultaneously assayed on two controls as well as on the active sensor.
Early analysis of non-specific binding to matrix proteins such as HSA or BSA can be critical. Additional in-line control not only halves the number of assay cycles required but can also improve data quality by eliminating potential variations between multiple sample preparations.
From initial assay development to pre-defined method templates, the Sierra SPR Control Software provides the perfect solution for every use case. It combines the flexibility of the hardware with an easy-to-use drag and drop method editor. A well-defined plate and method library supports personal workflow structuring and the data view allows real-time visual monitoring of flow cells.
Investigation of condition-dependent binding using frame inject
The traditional investigation of condition-dependent binding demands high reagent costs as well as lengthy run time. Frame inject delivers cost and time savings at low sample consumption.
How to influence ligand density using amine coupling
Finding the ideal immobilization level to obtain optimal kinetic measurements is critical. Here we examine the influencing parameters on different example targets.
Screening and characterization of small molecule binding to protein targets
Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of small molecule drugs and drug candidates.
Screening and characterization of biotherapeutics
Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of protein therapeutics.
For Research Use Only. Not for use in clinical diagnostic procedures.