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CryoProbes

TXO CryoProbe

Expanding the range of biomolecular NMR spectroscopy The family of triple resonance observe CryoProbes, optimized either for 13C or 15N detection, reveals new possibilities for studding the most challenging biological systems: intrinsically disordered proteins, large molecular complexes and in-cell NMR.

TXO CryoProbes with integrated cooled preamplifiers for 1H, 13C, 15N and 2H allows considerable progresses in 13C/15N direct detection for biomolecular NMR applications. In addition, observe probe geometry results in 1H channel being less affected by detrimental high-salt effects at higher field strengths. This makes TXO probes compatible, in terms of 1H sensitivity, to TCI probes  at physiological and higher salt concentration.

 In conjunction with the latest GHz-magnets and novel 15N direct-detect NMR methods, the CryoProbe makes direct 15N detection sensitive and advantageous in very large globular proteins and in IDPs due to the longer relaxation times, high resolution and low chemical shift anisotropy of 15N spectra.

Professor Gerhard Wagner of Harvard Medical School, a pioneer of 15N direct detection, stated: “Direct 15N and 13C detection methods have recently been evolved and found to be almost as sensitive as 1H detection techniques, benefitting from the slow transverse relaxation. 15N-detected TROSYs in particular do not require protein perdeuteration, which avoids the confounding problem of incomplete amide back exchange in large proteins that cannot be refolded and open avenues for expressing [13C,15N]-labelled proteins in insect cells or mammalian systems. The low-gamma NMR detection methods also provide new opportunities for studies of proline-rich polypeptides often found in regulatory regions, such as phosphorylation domains. However, spectra of such domains are typically poorly dispersed and highest field strengths will be needed to reveal mechanisms of phosphorylation-dependent switch mechanisms. Availability of GHz-class NMR instruments will be important for revealing mechanisms of phosphorylation-dependent signalling switches.”

Technical Details

  • •Highest sensitivity  for 13C or 15N direct detection
  • •Operating frequencies 400-1200 MHz (1H frequency)
  • •Four fully independent 1H, 2H, 13C and 15N channels with integrated cooled preamplifiers
  • •Simultaneous decoupling of up to three nuclei.
  • •Cooled 2H preamplifier for excellent stability
  • •Fitted with an actively shielded single axis Z-gradient for 5 mm sample diameters
  • •Standard sample temperature range -40°C – 150°C
  • •ATM and CryoFit compatible
  • •Probe of choice for the most challenging bio-NMR applications at 800 MHz and above

Service and Support

LabScape

Service & Life Cycle Support for Magnetic Resonance and Preclinical Imaging

Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.

LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory