TXO CryoProbes with integrated cooled preamplifiers for 1H, 13C, 15N and 2H allows considerable progresses in 13C/15N direct detection for biomolecular NMR applications. In addition, observe probe geometry results in 1H channel being less affected by detrimental high-salt effects at higher field strengths. This makes TXO probes compatible, in terms of 1H sensitivity, to TCI probes at physiological and higher salt concentration.
In conjunction with the latest GHz-magnets and novel 15N direct-detect NMR methods, the CryoProbe makes direct 15N detection sensitive and advantageous in very large globular proteins and in IDPs due to the longer relaxation times, high resolution and low chemical shift anisotropy of 15N spectra.
Professor Gerhard Wagner of Harvard Medical School, a pioneer of 15N direct detection, stated: “Direct 15N and 13C detection methods have recently been evolved and found to be almost as sensitive as 1H detection techniques, benefitting from the slow transverse relaxation. 15N-detected TROSYs in particular do not require protein perdeuteration, which avoids the confounding problem of incomplete amide back exchange in large proteins that cannot be refolded and open avenues for expressing [13C,15N]-labelled proteins in insect cells or mammalian systems. The low-gamma NMR detection methods also provide new opportunities for studies of proline-rich polypeptides often found in regulatory regions, such as phosphorylation domains. However, spectra of such domains are typically poorly dispersed and highest field strengths will be needed to reveal mechanisms of phosphorylation-dependent switch mechanisms. Availability of GHz-class NMR instruments will be important for revealing mechanisms of phosphorylation-dependent signalling switches.”
Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.
LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory