Detecting Reactive Oxygen and Nitrogen Species Using EPR – from Data Acquisition to Quantitative Analysis

Reactive oxygen and nitrogen species (ROS and RNS) are involved in a vast range of major diseases including cardiovascular disease, Alzheimer’s disease, Parkinson’s disease, cancer, multiple sclerosis, autism, and infections. These free radicals have an unpaired electron, creating a highly reactive molecule that will try to obtain an additional electron from wherever it can. In the cells and tissues of the human body this can result in major damage to lipids, proteins and DNA, ultimately affecting the function of cells, tissues and organs.

Some of the most abundant radicals produced in natural biochemical reactions are reactive oxygen species (ROS) such as hydroxyl, hydroperoxyl and superoxide radicals, and reactive nitrogen species (RNS), such as nitrogen monoxide and peroxynitrite.

In this webinar, Kalina Ranguelova, Applications Scientist for Bruker BioSpin, will outline how Bruker electron paramagnetic resonance (EPR) technology can be applied to the detection of ROS and RNS, focusing in particular on the use of spin trapping techniques.v

The webinar will highlight how Bruker’s EMXnano spectrometer, with its compact desktop footprint and easy-to-use functionality, makes this technology accessible to more labs and researchers than ever before.

What you will learn

This webinar will provide you with a background to how EPR works and spin-trapping techniques for analysing reactive oxygen and nitrogen species.

Key topics include:

  • What is EPR?
  • Reactive oxygen and nitrogen species
  • Spin-trapping and probe techniques
  • The Bruker EMXnano

Kalina Ranguelova was previously a researcher at the National Institute of Environmental Health Sciences where she studied EPR spin trapping and, since 2011, she has been an EPR Applications Scientist at Bruker BioSpin where she currently focuses on the analysis of beer, oil, wine, and pet food oxidation due to free radical processes.

During this webinar, she will outline how Bruker systems, including EMXnano can been applied to the detection and quantification of ROS and RNS.

The webinar will begin with an explanation of what EPR is and how it works and will touch upon its advantages over other techniques for detecting reactive species, such as chemiluminescent and fluorescent probes or assay-based methods.

Dr Ranguelova will then go on to discuss the methods of spin trapping and spin probes, approaches which help to overcome the short half-life of free radicals at room temperature and allow data on these reactions to be captured.

And by focusing on the EMXnano, Bruker’s most user-friendly desktop EPR instrument, Dr Ranguelova will highlight that EPR is a method that can be used by virtually anyone, with very little knowledge of EPR required.

Who should attend

This webinar will interest anyone who wants to know more about the use of EPR for detecting ROS and RNS, including those who are coming to EPR for the first time. It is likely to be of particular interest for biologists, chemists, biophysicists and those working in academic research settings and pharmaceutical research and development.


Dr. Kalina Ranguelova

Senior EPR Applications Scientist, Bruker BioSpin

Dr. Kalina Ranguelova is an EPR Applications Scientist in Bruker BioSpin Corporation since 2011. She completed her Ph.D. at The Bulgarian Academy of Sciences where she received a Ph.D. with research focused on inorganic copper complexes structure using electron paramagnetic resonance (EPR) spectroscopy. After two research positions at CUNY and National Institute for Environmental Sciences where she studied free radical biology and EPR spin trapping as method for measurement of reactive oxygen species (ROS), she joined Bruker and holds a role as Applications Scientist. Her current focus is detection and identification of free radicals in biological systems and pharmaceuticals using spin traps and spin probes. She has publications in journals like Journal of Biological Chemistry, Biochemistry, Free radical Biology and Medicine, etc. She has presented in many international meetings related to free radical research in biology and protein chemistry.

Dr. Ralph Weber

Senior EPR Applications Scientist, Bruker BioSpin

Dr. Ralph Weber started his scientific training at Brown University where he received a B.A in Chemistry and German Literature and Language. He continued his training at the University of Chicago, earning a Ph.D. in chemistry focusing on EPR and ENDOR studies of proteins and lanthanide complexes. Two postdoctoral positions followed. At Leiden University in the Netherlands he studied excited states of molecules using ODMR (Optically Detected Magnetic Resonance) and designed and constructed a high frequency pulse EPR spectrometer. At MIT he studied motional dynamics in lipids via solid state NMR and was one of the original project members to design and construct a DNP (Dynamic Nuclear Polarization) spectrometer incorporating a high power gyrotron. He joined Bruker 29 years ago in 1989. He is responsible for much of the documentation for EPR and also offers customer support for pulse, high frequency, and imaging applications. He is currently co-principal investigator on a five-year NIH grant to develop pre-clinical EPR imaging technology and to promote its use in the pharmaceutical industry.