MicroProbes (1mm TXI, 1.7mm TXI) - Small volume probes for the analysis of quantity limited samples

Over the past decades there has been a significant growth in demand for miniaturization in all areas of modern research and development. Evoked by many exciting applications, there is a need for analytical methods which require smaller amounts of sample.

Bruker meets this challenge with a revolutionary NMR probe design: The 1mm (and 1.7mm) MicroProbe. It operates with disposable 1mm (resp. 1.7) capillary sample tubes and the sample volume of 5 (resp. 30) microliters enables use of the lowest amounts of sample to run all high resolution NMR experiments with outstanding sensitivity.
Due to the TXI-type probe design, the z-gradient coil and the automatic matching and tuning accessory, the 1mm MicroProbe can be used for a wide variety of NMR experiments.

The 1mm TXI MicroProbe offers the highest 1H mass sensitivity for RT probes which results in an up to 4 times higher mass sensitivity than 5mm conventional probes (with respect to the same sample amount). The 1.7mm TXI MicroProbe offers an optimal 1H and 13C mass sensitivity.

Technical Details

  • 1mm TXI MicroProbe: up to 4 times higher mass sensitivity than 5mm conventional probes (with respect to the same sample amount)
  • 1.7mm TXI MicroProbe: an optimal 1H and 13C mass sensitivity
  • Excellent solvent suppression properties
  • Virtually no salt effect
  • Discrete samples in tubes that can be sealed and stored
  • Automation accessory for sample preparation and handling available
  • ¹H, ¹³C and ¹⁵N channel
  • Very short pulses on all channels

The sample tubes are transferred into the magnet by use of shuttle, very similar to the well known 5mm spinners. The tube does not need to be adjusted inside the shuttle since all adjustments are made automatically inside the probe. These shuttles may be used in Bruker-BioSpin’s automatic NMR sample changers such as the SampleCase and the SampleJet.


The MicroProbes works with discrete, disposable 1mm sample tubes, which may be sealed after filling. The glass tubes have a plastic, v-shaped top part attached to them, which allows for easy manual sample filling even with volumes as small as 5 (resp. 30) micro liters. The tubes may be equipped with a digital matrix code on the plastic top parts, which then uniquely identifies each sample.
Even though manual sample filling is straight forward, with the SamplePro, Tubeᵀᴹ a sample preparation robot is available, which enables the fast, reliable and comfortable filling of many 1mm (resp. 1.7mm) sample tubes at a time. Here the sample tubes are arranged in the industry-standard 96 well plate format, so that the tube racks are fully compatible with all other laboratory or storage equipment which is capable to handle this format. They can also be directly used in our SampleJet.



  • Excellent solvent suppression, with only a small amount of solvent in the active volume
  • Virtually no salt effect, due to the small tube  diameters
  • Very short pulses for all nuclei, opening up an exciting new range of applications
  • Discrete sample tubes:
    • Funnel for manual filling, or can be filled automatically
    • Cap to protect the sample
    • Automated sample identification with 2D matrix codes



Mass Limited Samples and Reduction of Expenses for Deuterated Solvents

Due to their high mass sensitivity MicroProbes are especially well suited for the investigation of highly soluble compounds that are only available in limited amounts. This is often the case in natural products, but also in body fluids like CSF or blood and urine from rodents. The TXI-probe design allows to run the full range of experiments typically needed in biologic samples.

Needing only small amounts of deuterated solvents helps to keep the overall cost of an experiment low and will also help to get best performance, if solvent suppression is required. Therefore MicroProbes are well established in quality control of compound libraries, where only limited quantities of protonated solutions are available for NMR spectroscopy.

The small sample volume required results in short pulses that are hardly influenced by the ionic strenght of the sample, making these Probes very attractive for salty samples, like urine.

  • Natural products
  • Biofluids (urine, blood, CSF)
  • Quality control of compound libraries
  • Routine spectroscopy of small organic compounds
  • Solvent suppression
  • Salty samples


Service and Support


Service & Life Cycle Support for Magnetic Resonance and Preclinical Imaging

Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.

LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory