FT-IR/Raman Training Course



The FT-IR/Raman Training Course is designed for those users new to data acquisition and sample preparation. Laboratory teaching emphasizes measurement using various sampling accessories and spectral manipulations with the OPUS software. Three optional special topics are given on Thursday on specific advanced techniques.

The course will consist of lectures, laboratories sessions, and demonstrations which emphasize basic principles of FT-IR instrumentation, data processing, sampling techniques, the OPUS/IR PC software, and introductory instrument maintenance and troubleshooting.


  • Overview of NIR
  • Introduction to Matrix-F, process probes and accessories
  • Best practices for installation and operation of inline equipment
  • CMET process software
  • Sampling theory
  • Process (I/O) communications
  • Instrument diagnostics and maintenance
  • OPUS/CMET Software
  • Basic maintenance procedures
  • Discussion with service personnel


A working knowledge of the Windows XP or Windows 7 operating system and a familiarity with your Bruker FT-IR and FT-Raman instrument. The basics aspects of the operating system (e.g. copying files, formatting disks, installing printers) can be practiced in the tutorials provided with the operating system.

Please bring your laptop with OPUS software installed if available.


March 15th - 18th, 2021
June 7th - 10th, 2021
September 13th - 16th, 2021
December 6th - 9th, 2021


Training course syllabus


1)  Introduction to FTIR spectroscopy:

  • Molecular vibration and light absorption
  • Interferometer: constructive and destructive interference
  • Interferogram of monochromatic, polychromatic and broadband sources
  • Advantages of FTIR spectroscopy
  • Principles of data processing: folding, apodization, zerofilling; phase errors
  • Spectral resolution

2)  Optical components of FTIR spectrometer:

  • Sources
  • Beamsplitters
  • Detectors
  • Windows
  • Mirrors
  • Polarizers
  • Optical filters

3)   FTIR Sampling Techniques:

  • Transmission
  • Reflectance
  • ATR

4)   Spectral Interpretation:

  • Alkanes, phenols, ethers, ketones, acids, etc.

5)   FTIR test protocols:

  • Validation test setup
  • Running OVP tests

6)   Instrument care:

  • Basic maintenance and troubleshooting
  • Optical components exchange


1)   Introduction to Raman:

  • Infrared vs. Raman spectroscopy
  • Dependency of the excitation line
  • Spike correction
  • Fluorescence and thermal background

2)   OPUS I: Basic functions and data acquisition:

  • Software overview
  • File structure
  • Working with files
  • Setting experimental parameters

3)   Lab session I, data acquisition:

  • Effect of spectral resolution
  • Effect of apodization function
  • Effect of zerofilling
  • Effect of number of scans and S/N evaluation

4)   OPUS II: Data manipulation and evaluation:

  • Base line correction
  • Spectral subtraction
  • Library search
  • Plotting spectra
  • Quantitative analysis

5)   Lab session II, data manipulation:

  • Exporting data
  • Peak picking
  • Plotting with peaks
  • Baseline correction
  • Spectral subtraction
  • Plot layout editor


1)   Lab session II continued

2)   OPUS 3D:

  • 3D file structure
  • Chemical image
  • Extract spectra

3)  OPUS 3D lab session

4)  Quantitative analysis:

  • OPUS Quant I and II
  • Selecting of samples
  • PLS data modelling

Optional sessions (Thursday)

1)   Rapid scan and step-scan time-resolved spectroscopy:

  • Principles of rapid- and step scan FTIR
  • Components: detectors, digitizers, filters
  • Parameters optimization
  • Triggering
  • Examples
  • Lab demonstration

2)   Microscopy:

  • Overview
  • Sample visualization
  • Sample preparation
  • Transmission, reflectance, ATR and grazing angle techniques
  • Resolution
  • Mapping and imaging
  • Lab session

3)   Raman spectroscopy:

  • Principles of Raman spectroscopy
  • Side effects: fluorescence and thermal emission
  • Selection of excitation laser
  • FT vs. dispersive Raman spectroscopy
  • Raman microscopy
  • Lab session

Further Information


The core course will run from Monday to Thursday with the option to sign up for one-on-one sessions on Friday.


We will be serving lunch during the training courses, if you have any food allergies or special restrictions, please let us know in advance.

Hotel & Transportation:

Bruker Optics has special rates with the local hotels, upon your registration, we'll contact you with confirmation and provide you more details.


$ 1800

(includes course materials)

If your Bruker FT-IR or FT-Raman spectrometer has been installed within the last 12 months, one tuition is normally included as part of your purchase (please refer to your system quotation).