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Intra- and extracellular real-time analysis of perfused fibroblasts using an NMR bioreactor: A pilot study
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Metabolomic discrimination of various mitochondrial defects presents a significant challenge. This study describes an NMR-based bioreactor system that enables real-time investigation of intra- and extracellular metabolism in perfused fibroblasts.
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Christian Urzì, Christoph Meyer, Déborah Mathis, Peter Vermathen and Jean-Marc Nuoffer
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Journal of Inherited Metabolic Disease
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09/2024
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Determination of Intra- and Extracellular Metabolic Adaptations of 3D Cell Cultures upon Challenges in Real-Time by NMR
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This study presents a perfused NMR bioreactor system enabling real-time, quantitative analysis of intra- and extracellular metabolites in human fibroblasts. Using diffusion NMR, 35 metabolites were detected, with 19 successfully separated by compartment. The approach demonstrates sensitivity to metabolic changes under different media and flow rates.
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Christian Urzì,Damian Hertig, Christoph Meyer, Sally Maddah,Jean-Marc Nuoffer and Peter Vermathen
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International Journal of Molecular Sciences
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06/2022
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Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy
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This study presents a reproducible NMR bioreactor system for simultaneous 1H-NMR metabolite profiling and 19F-NMR oxygenation monitoring in 3D collagen-based cell cultures. A standardized setup enables dynamic assessment of mitochondrial respiration and metabolic flux under perfusion.
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Damian Hertig, Sally Maddah,Roman Memedovski,Sandra Kurth, Aitor Moreno,Matteo Pennestri, Andrea Felser, Jean-Marc Nuoffer and Peter Vermathen
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Analyst
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06/2021
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Real-time Metabolic Investigations of Living Cells by NMR – First Experiences
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This study demonstrates the feasibility of real-time metabolic monitoring in 3D cell cultures using the commercially available InsightCell™ tool, enabling reproducible glucose and oxygen measurements over 12 hours. Metabolic interventions triggered rapid, reversible shifts between aerobic respiration and anaerobic glycolysis.
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Damian Hertig, Debora Felser, Gaëlle Diserens, Sandra Kurth, Jean-Marc Nuoffer and Peter Vermathen
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Magnetic Resonance in Medicine
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05/2019
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