Scientist working with cell cultures

Stability Testing for PTMs via Intact Analysis

  • Rapid screening of a biotherapeutic degradation products during storage
  • Reduced risk of artifact introduction compared to peptide maps
  • True isotopic patterns provide accurate quantitation 
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Stability Testing for PTMs on a maXis 4G
Data acquired on maXis 4G

In this example the allergen rBet v 1 protein was analyzed at the intact level before and after storage.

A 15.999 Da mass shift was rapidly identified which corresponded to a detrimental oxidation of Methionine as a result of storage conditions of the product.

The incredible raw data delivers True Isotopic Patterns which are essential for accurate quantitation.

Subunit analysis for oxidation is much less susceptible to artifacts than peptide mapping due to reduced analysis time and fewer handling steps.