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ESI-ITMS

amaZon speed ETD

The new amaZon speed ETD incorporates key inventions in ion trap mass spectrometry for unprecedented proteomics performance.

Ion trap innovation

Market-leading dynamic range

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ESI-ITMS

amaZon speed ETD

Sensitivity
Leading dual ion funnel technology offers unparalleled sensitivity.
Confidence
Market-leading mass accuracy ensures high confidence in identification.
Speed
Unrivaled MS/MS scan speed unravels the complexity of proteomics samples.

Características

amaZon speed ETD

Unmatched ion trap performance for proteomics solutions

The amaZon speed ETD incorporates key inventions for unprecedented proteomics capabilities. The high dynamic range and complexity of proteomics samples due to post translational modifications require maximum speed and sensitivity to capture as many peptides as possible.

The amaZon speed ion ETD trap sets new standards in protein identification due to its enhanced MS/MS speed, new SWIFT isolation and SMART fragmentation.

This results in routine identification of more than 2500 unambiguously identified proteins in a single LC-MS/MS run from 5 µg of a HeLa lysate. The dual ion funnel transfer line is delivering absolute benchmark sensitivity.

Beneficios

amaZon speed ETD

Unmatched ion trap performance through innovation

amaZon speed ETD offers:

  • Bottom-up proteomics identifying more low abundant proteins due to its greatly increased MS/MS duty cycle
  • Top-down sequencing (TDS) for the highest protein sequence coverage
  • Market leading mass accuracy for confidence in results
  • Leading dual ion funnel technology for unparalleled sensitivity
  • Outstanding ETD/PTR applications for full post-translational modification (PTM) analysis
  • SMART isolation and fragmentation
  • Unrivaled scan speed of 52,000 u/sec at a peak width < 0.5 u for resolution of doubly charged ions
 
 
 

High sequence coverage by CaptiveSpray
The amaZon speed is equipped with the high-transmission, robust CaptiveSpray source for high performance and reproducibility in bottom-up studies.

Ultimate protein identification rates
The amaZon speed offers a survey view of a Hela cell digest (5 µg) separated by a nano UHPLC and amaZon speed ETD equipped with a CaptiveSpray ion source.

Market leading dynamic range
The amaZon speed ETD identifies proteins over at least five concentration levels in a single run.

Aplicaciones

amaZon speed ETD

Complete PTM characterization by Electron Transfer Dissociation (ETD)

Many proteins execute their molecular function by reversible posttranslational modifications (PTMs), like phosphorylation for example. The identification and unambiguous localization of the modification site is of major importance to understand the biological context. ETD preserves the modification of the peptide backbone and therefore allows clear assignment of the modification site. The amaZon speed offers unrivaled performance through its proven sensitive, robust and reliable ETD technology.

Glycopeptide analysis

Understanding relationships between structure, location, and function of glycoproteins requires detailed information about peptide sequence, glycan composition, and glycosylation sites. A wide variety of analytical approaches are employed to analyze intact glycoproteins, glycopeptides, and released glycans, with mass spectrometry playing a major role.

The performance of the amaZon speed ETD system for the analysis of N-glycosylated peptides in simple glycoprotein digest using Collision Induced Dissociation (CID) and Electron Transfer Dissociation (ETD) has been demonstrated.

The efficient combination of CID and oxonium-ion–triggered ETD during data acquisition – “Fragment Triggered ETD” – enables extensive protein sequence identification and detailed glycopeptide profiling in a single run, even in complex mixtures.

Combining the CaptiveSpray nanoBooster ionization source and Fragment Triggered ETD on the amaZon speed ETD platform greatly enhances glycopeptide analysis in protein mixtures.

Charge distribution and intensity of two different N-glycopeptides measured by Captive Spray and nanoBooster; A: CRPINATLAVEK and B: NVTSESTCCVAK

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