On-Demand Session: Star Light, Star Bright: Considerations for Imaging Astrocyte Dynamics
Investigate the role of astrocytes during sleep with 1P and 2P microscopy
Star Light, Star Bright: Considerations for Imaging Astrocyte Dynamics
PRESENTATION HIGHLIGHTS:
[00:03:26] Intro to Dr. Ashley Ingiosi and their interest in astrocytes and sleep
[00:04:45] Insufficient sleep is a prevalent health issue
[00:12:03] Challenges for studying astrocytes
[00:13:29] In vivo Ca+2 imaging of astrocytes
[00:21:00] Research questions and answers
[00:28:20] Conclusions on Ca+2
[00:29:31] Challenges and solutions encountered during imaging and analyses
[00:37:22] Analysis considerations for spontaneous sleep-wake behavior
[00:45:44] Take-home points
[00:46:22] Acknowledgments
