DISCOVER a screening assay for molecular glues and PROTACs: ternary complex analysis with induced proximity
Webinar Overview
In this episode of the Bruker Biosensors DISCOVER MOLECULAR INTERACTIONS webinar series you will learn how to analyze binary and ternary binding kinetics of bispecific molecules like PROTACs.
Key Learning Objectives
This webinar is part of the webinar series DISCOVER MOLECULAR INTERACTIONS by Bruker Biosensors.
Proteolysis-targeting chimeras (PROTACs) and molecular glues are novel pharmaceutical compounds inducing the degradation of a target protein. This mode of action depends on the formation of a ternary complex with two proteins. Ternary complexes are influenced by complex cooperativity and avidity effects, which makes the kinetic characterization challenging.
In this episode, we will showcase a proximity binding assay for the simultaneous measurement of binary and ternary interaction kinetics in high throughput using a Y-shaped DNA nanostructure. The assay is applied to cereblon and von Hippel-Lindau as E3 ligase substrate receptors. We will showcase results from a screening of a range of compounds and benchmark the approach against data from gold-standard solution techniques.
Speaker
Alice Soldá, Ph.D.
Head of Application Specialists, Bruker Biosensors, Munich, Germany
Recording
For Research Use Only. Not for use in clinical diagnostic procedures.