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Webinar Recap: Imaging Breast Cancer Evolution in 3D Organoid Cultures with Luxendo Light-Sheet Microscopy
In this webinar recap, Dr. Martin Jechlinger, the Senior Scientist and Head of VISION Laboratory at the MOLIT Institute, talks about how his lab utilizes light-sheet microscopy to investigate 3D organoid cultures. Specifically, the molecular mechanisms underlying mammary tumor development during breast cancer, as well as what is happening when treatments are failing.
In our "Imaging Breast Cancer Evolution in 3D Organoid Cultures with Luxendo Light-Sheet Microscopy" webinar, Dr. Jechlinger provides an in-depth look at his research exploring the formation, regression, and relapsing of mammary tumors and his team's development of an improved stochastic model of breast cancer.
Dr. Jechlinger's presentation covers his lab's methods and experimental setup, their findings — including single-cell tracking data — and his team's progress toward answering crucial questions about cellular behaviors and the rates at which and reasons why they proliferate into tumors within an organoid.
Audience Q&A and Other Answers
Download the webinar recap or watch the recording to learn more about these and other topics.
- Why use light-sheet technology for this type of research?
- How can your team's findings be used to address targeted breast cancer therapies?
- Is the inverted SPIM system compatible with multi-well plates? Did you use a multi-well plate in these experiments?
- Did you find local differences in the oncogene tension in the spheroid? If so, is the area of low oxygen more associated with proliferation?
- Does proliferation correlate with malignation? If so, do you see EMT?
- Do you see similars trends in the normal cells, tumor cells, and residual cells in both the animal model and the human samples? If so, what future experiments will this inform?