nVista 2P
nVista 2P
The nVista 2P miniscope utilizes two-photon technology to perform neural circuit imaging at higher resolution and greater depths than one-photon techniques during freely-behaving tasks. This high-performance fluorescence imaging solution weighs only 2.2 grams and enables researchers to target all regions of a rodent’s brain using the same techniques as one-photon experiments, but with the additional benefits of two-photon optics.
Innovative Design
The nVista 2P system is a compact miniscope that weighs only 2.2 grams. Its unique lightweight and well-balanced footprint allows freely-behaving experiments without compromising the animal’s ability to interact with its environment. The nVista 2P hardware is seamlessly integrated into an innovatively designed mobile workstation, providing researchers the ultimate flexibility and convenience of bringing the instrumentation directly to their experimental setups. The full system includes:
- Fully integrated platform: Including laser, DAQ, and computer
- Mobile workstation: This ergonomic cart is designed for easy setup and transport to your experiment
- nVista 2P miniscope camera: The smallest and lightest miniscope camera commercially available for two-photon imaging
Enhanced Behavior Studies
The nVista 2P design brings the benefits of two-photon optics to your behavioral studies. Traditional benchtop systems often need workarounds to recreate specific behaviors, such as joystick and ball running, which require extensive training and can lead to unrealistic behaviors. The nVista 2P solution facilitates two-photon freely-behaving experiments without compromise. When paired with the Inscopix nVision behavior recording system, scientists can now more tightly correlate such natural behaviors as place preference and socialization with neural activity.
Complete Ecosystem
Optimized to accelerate imaging success, the nVista 2P workflow leverages Bruker’s full suite of innovations, such as ready-to-image (RTI) viruses and the ProView Express Kit, which simplify surgical workflows and downstream data analysis through the well-known Inscopix Data Exploration, Analysis, and Sharing (IDEAS) platform. This end-to-end environment helps great science happen faster with complete tools and elite scientific support for data acquisition, analysis, and even grant submission.
In Vivo Calcium Imaging During Free Behavior
With a field of view (FOV) of 400 x 400 microns and the ability to focus over 300 microns into tissue, the nVista 2P camera’s versatile design is compatible with cranial window and GRIN lens preparations. The miniscope is also compatible with well-known analysis solutions used in one-photon and two-photon imaging, allowing researchers to confidently assess data quality, run statistical inferences, and even parse differences within cell populations across annotated behavioral or neuronal epochs.
Observe Vascular Activity
The nVista 2P miniscope retains the versatility of Bruker one-photon systems with the ability to image non-neuronal, fluorescence-like blood vessels. The expanded vessel diameter estimations analysis package available in both IDPS and the IDEAS platform allows researchers to directly study vascular reactivity in relation to behavioral and pharmaceutical challenges. This will help researchers tackle major questions in cardiovascular health and disease prevention.
nVista 2P Specifications
| Description | Specifications |
| Modality | Single-channel two-photon |
| Integrated System with Laser | Yes |
| Weight | 2.2 g |
| Field of View | 400 μm x 400 μm |
| Objective Lens NA | 0.5 |
| XY Resolution | 1.6 μm |
| Z Resolution | 15.7 μm |
| Electronic Focusing Range | 300 μm |
| Frame Rate | 15 Hz at full FOV; 30 Hz at half FOV |
| Behavior Synchronization | nVision and TTL |
| Image Size | 512 x 512 pixels |
| Data Cable | 2.3 m |
| GPIO and Port | 1 SYNC and 1 TRIG TTL; 2 Ethernet ports (nVision, Network); 1 USB |
| Storage | 4 TB |
| Laser Characteristics | |
| Wavelength | 920 nm, fixed |
| Average Power | 150 mW |
| Pulse Width | <120 fs |
| Repetition Rate | 80 MHz ± 2 MHz |
| Cart Characteristics | |
| Height | 152 cm |
| Length | 56 cm |
| Width | 58 cm |
| Weight | 102 kg |
nVista 2P Specifications
| Laser Characteristics | |
| Modality | Single-channel two-photon |
| Integrated System with Laser | Yes |
| Weight | 2.2 g |
| Field of View | 400 μm x 400 μm |
| Objective Lens NA | 0.5 |
New! Miniscope Accessories Online Store
At the core of every successful calcium imaging study is precise expression and optical access. Bruker offers a comprehensive portfolio of consumables to support your Inscopix miniscopes research, including Gradient Refractive Index (GRIN) lenses and workflow-enhancing kits. These solutions are designed to simplify experimental setup and enable high-resolution imaging in deep brain regions of freely behaving animals. Discover how our reagents and optics can accelerate your path from surgery to insight.
