KEYWORDS: Optical Tweezers; NanoTracker; Fluorescence Microscopy; Multi-Fluorescence Microscopy; Optical Integration; Optical Trap; Force Measurements
Fluorescence microscopy and optical tweezers are two useful tools across disciplines in the life sciences. One of the few instruments that is commercially available that combines both techniques is the JPK NanoTracker.
In this technical note, it is demonstrated that modifications performed by Bruker to an inverted microscope do not interfere with fluorescence or any other light microscopy technique. The two beam paths are not interfering with each other, which makes it possible to use different kinds of microscopy techniques at the same sample position and with the same trapped beads. Also, the possibility to use multi-fluorescently labeled samples and change the excitation and emission filters during operation is provided.