Non-Uniform Sampling (NUS) is an acquisition method for NMR experiments with two or more dimensions. It skips a random fraction of data that would usually be measured and so saves acquisition time. Reconstruction algorithms are used to get a correct spectrum from such sparse data set.
NUS is especially useful for multi-dimensional experiments in biomolecular NMR, where time savings up to a factor of 10 and even more in 4D and 5D experiments can be achieved. With NUS, new protein structure determination experiments become feasible.
Small molecule applications based on 2D spectra can also benefit from an accelerated acquisition. Improvements in spectral resolution and the quality delivered by NUS without the need to increase the overall acquisition time are the main reasons for it’s usage.
Non-Uniform Sampling (NUS) is fully integrated into TopSpin’s acquisition and processing. It allows routine use and is available for all kinds of NMR experiments. An automatically generated and optimized NUS sparse list defines the sampling pattern leading to the fractional data acquisition. Multi-dimensional decomposition then allows for the calculation of the missing data points, enabling a regular Fourier transform processing of the complete data set, all done in a fully automated fashion.
The setup and execution of NUS experiments is covered by the TopSpin license. This also includes the processing of 2D spectra. Processing of 3D – 5D NUS spectra requires an additional license.
NUS implementation in TopSpin results from a research collaboration with Prof. Orekhov and co-workers.
Orekhov, V.Y., I. Ibraghimov, and M. Billeter, J. Biomol. NMR, 2003. 27(2):p. 165-173
M Mayzel 1, K Kazimierczuk, V Yu Orekhov, Chem Commun (Camb). 2014 Aug 18;50(64):8947-50.
Krzysztof Kazimierczuk 1, Vladislav Yu Orekhov, Angew Chem Int Ed Engl. 2011 Jun 6;50(24):5556-9.
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