Training

Bio-NMR

MR for biological molecules

BioNMR

This 1-day course covers the set up and processing of experiments commonly used for structure determination of biological macromolecules. Discussions include spectrometer configurations, the calibration of pulses, parameter optimization, and acquisition of 1D, 2D and 3D experiments. Data processing of 3D data, including linear prediction, phase corrections, strip transforms, and baseline corrections will also be covered. The lab session uses extensively the bioTop program in Topspin for automatic acquisition and processing.

Experiments covered include:

2D Heteronuclear Correlation Experiments (¹H-¹⁵N HSQC, ¹H-¹³C HSQC)
3D Experiments (NOESY-HSQC, TOCSY-HSQC and several triple resonance experiments HNCO, HNCACB, CBCA(CO)NH etc.)
Pseudo-2D Relaxation Experiments with automatic analysis by the Dynamics Center