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NeuraLight 3D

The most advanced 3D holographic workstation for simultaneous all-optical stimulation and imaging

Spatial Light Modulation (SLM) for Ultima Microscopes

The Ultima NeuraLight 3D is the next step forward in the use of optogenetics in functional paradigms to study and understand neural connectivity and how neural networks function. In close collaboration with leaders in neuroscience research at Stanford University, Columbia University, and University College London, Bruker has developed the next major technological breakthrough to complement the ever evolving Ultima family of multiphoton microscopes.

3D Optical Stimulation and Multiphoton Imaging Combined

Titrate precise, simultaneous, multicell stimulation in a 3D volume for in vivo studies

The Ultima NeuraLight 3D SLM (spatial light modulator) leverages the flexibility of Ultima’s modular platform design, along with new licensed technology, to create 3D laser holograms to simultaneously stimulate cells in three dimensions. Coupled with a set of 6-millimeter galvanometers, the NeuraLight 3D SLM enables multiple stimulation points in either spiral or rectangular scan patterns to be projected in a 3D volume.

Perform multiplexed diffraction-limited subcellular photoactivation

NeuraLight 3D can also generate precise, diffraction-limited points in a 3D space for subcellular resolution. Stimulation patterns targeting individual synapses or groups of synapses can be produced, allowing researchers to probe neural connectivity and signaling. Sequencing of stimulation patterns enables researchers to automate studies examining signal summation in individual neurons or in groups of neurons.

Optical stimulation of selected cells containing GCaMP6s using NeuraLight 3D. Cell bodies were stimulated simultaneously by creating a 3D hologram of points with the SLM and spiral scanning all points over the cell body. Courtesy of Adam Packer, Lloyd Russell, Henry Dalgleish, and Michael Hausser (University College London).

Target Functional Ensembles of Neurons

Rapidly switch between stimulation patterns.
The fast switching time of the NeuraLight 3D SLM allows users to rapidly change stimulation patterns. For in vivo studies, this provides a tool to mimic neuronal firing produced by behavioral stimuli. For subcellular studies examining connectivity and signaling, complex signal summation studies can be quickly executed.

Generate 3D activation patterns while imaging and recording electrical signals.
The module includes a complete software toolkit for generating 3D activation patterns while imaging/recording electrical signals and triggering behavioral and electrical stimuli. A calibration wizard makes calibration a simple and straight-forward process. 3D point location is mapped using an acquired Z series of the specimen. This also allows synchronization with imaging (resonant scanning, scanning line scanning, and point detection), along with electrical recording and triggering of other stimuli, including electrical and behavioral stimuli.

Using the NeuraLight 3D SLM, multiple targeted spots are generated and then spun in controlled spirals, creating a swinging spot grid for simultaneous optical stimulation and imaging.