Array of eight channels/four spots enables 13,200 interactions in 24 hours
Breakthrough SPR Detection for Sensitivity
The SPR+ detector derives its sensitivity from combining imaging surface plasmon resonance (SPRi) with a high intensity laser light source and high-speed optical scanning. This arrangement supports extremely sensitive imaging of relatively large two-dimensional sensor arrays, while the intensity of the light source permits the use of a high-speed camera, which in turn collects more resonance measurements per scan. The net result is a signal-to-noise ratio of 0.02 RU (RMS) and improved accuracy when measuring small response changes, which are frequently observed for fragment or small molecule binding experiments.
With 32 detection spots available during each analysis cycle, several user-defined configurations are possible to maximize high-throughput SPR analysis. This can include 31 control-subtracted injections, up to 3 in-line controls or 4 targets per channel. The autosampler is compatible with a wide range of 96- and 384-well microtiter plates and the dual-plate sample deck with common rack position allows for increased automation.
When equipped with an optional plate handling robot, the instrument can function in high-throughput mode and can analyze more than 4400 samples per day, resulting in more than 13,200 control-subtracted interactions.
Sierra SPR®-32 Pro can be easily integrated into an automated scheduling software via API.
The eight syringe continuous flow buffer is a powerful assay development and optimization tool. It also supports high-throughput SPR analysis under a range of conditions, including different pH, salt, detergent or solvent concentration. Simultaneous multi-buffer analysis saves time and reagents, while increasing information throughput.
The Sierra SPR®-32 Pro system is a robust instrument designed for high-throughput applications such as epitope characterization and antibody, fragment or small molecule screening.
Simultaneous injection of up to eight samples facilitates high-throughput assay development and optimization, as well as rapid quantitative analysis of both crude and purified samples. Hydrodynamic isolation (HI) continuous flow microfluidic technology addresses samples as flowing streams onto the sensor array. This allows processing of 8 samples on a standalone system, which can be boosted to more than 4400 samples per day through optional plate handling robotics.
Individual needle control (INC) optimizes the performance, flexibility and scope of applications, while saving time and materials when throughput is not the main requirement.
Up to eight buffers can be used simultaneously in the instrument, using frame inject. The ability to evaluate different pHs, ionic strength, detergent or solvent concentration, as well as the effect of co-factors or inhibitors, in a single assay results in simpler, optimized assays that ultimately increase throughput and productivity. The four-sensor per channel design provides maximum flexibility for control analysis, while maintaining throughput for the most complex analyses.
Four independently addressable detection spots are configured in each of the eight channels of the Sierra SPR®-32 Pro system. Samples can be simultaneously tested on up to three control surfaces as well as the active surface. In many SPR applications, early detection of nonspecific binding to control proteins is critical.
In small molecule screening and characterization, binding to serum proteins such as HSA and BSA is an integral part of drug development. The additional in-channel control halves the number of assay cycles required and improves data quality by eliminating potential variations between multiple sample preparations.
The SPR+ detector combines imaging SPR (SPRi) with a high intensity laser light source and high-speed optical scanning. The result is improved signal-to-noise and improves accuracy when measuring small response changes.
From initial assay development to pre-defined method templates, the Sierra SPR control software provides the perfect solution for every user. It combines the flexibility of the hardware with an easy-to-use drag and drop method editor. A well defined plate and method library supports personal workflow structuring and the data view allows real-time visual monitoring of flow cells.
Tackling Challenging Targets with High-Throughput Biophysical Screening at Novartis
Learn in this note how multiplexing, automation and automated data analysis with Genedata Screener take SPR to the next level in this real-life example.
How to influence ligand density using amine coupling
Finding the ideal immobilization level to obtain optimal kinetic measurements is critical. Here we examine the influencing parameters on different example targets.
Screening and characterization of small molecule binding to protein targets
Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of small molecule drugs and drug candidates.
Screening and characterization of biotherapeutics
Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of protein therapeutics.
Investigation of condition-dependent binding using frame inject
The traditional investigation of condition-dependent binding demands high reagent costs as well as lengthy run time. Frame inject delivers cost and time savings at low sample consumption.
For Research Use Only. Not for use in clinical diagnostic procedures.