On-Demand Session: Spatiotemporal Imaging with Light-Sheet Microscopy: Having Your Cake and Eating It Too
Learn how light-sheet microscopy enhances our understanding of molecular biology
Spatiotemporal Imaging with Light-Sheet Microscopy: Having Your Cake and Eating It Too
PRESENTATION HIGHLIGHTS:
[00:02:37] Intro to SPIM and Imaging FCS
[00:07:22] Simultaneous spatiotemporal resolution - overview and use cases
- [00:18:46] 3D diffusion coefficient maps
- [00:14:57] Measuring interactions
- [00:18:25] Spatial correlations
[00:32:00] Improving evaluation speed with deep learning and direct camera read-out
[00:35:10] Limitations of SPIM-FCS
[00:36:54] The future of multiparametric quantitative bioimaging with SPIM-FCS
Q&A
QUESTIONS:
- [00:00:19] How do you calculate correlation functions with SPIM-FCS?
- [00:01:14] What experiments would you suggest for someone new to FCCS?
- [00:02:07] Can you image two regions of interest (ROIs) at the same time?
- [00:02:44] How deep can you go into a tissue before artifacts or light distortions would be a problem?
- [00:04:14] Have you ever worked with SPAD arrays for this technique?
- [00:04:48] Do you acquire 3D diffusion coefficient maps sequentially?
