Fungal Infections

Diagnostic tools for fast identification and susceptibility testing in fungal infections

Основные моменты

Fungal infections – the clinical problem

The occurrence of invasive fungal infections has risen in recent years, and is compounded by the difficulty of early and accurate diagnosis and appropriate use of antifungal therapy. The majority of serious fungal infections occur in hospitalized patients and those with compromised immune systems. Outbreaks of multi-drug resistant (MDR) fungal infections are therefore commonplace in the healthcare environment across the world.


The identification of molds and multicellular fungi to the species level continues to be one of the most challenging aspects of microbiology, and hinders the timely delivery of life-saving treatment. However, recent advances in diagnostic tools are changing the outcome of fungal infections worldwide.

Rapid, culture-free detection

Traditional diagnostic methods for fungal infection include direct microscopic examination of clinical samples, histopathology, culture and serology. More recently, analytical tools such as mass spectrometry (MS) and molecular diagnostics have gained popularity in clinical laboratories.

If the sample has already been cultured, Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) MS, such as the MALDI Biotyper®, provides reliable, accurate identification of fungi down to the species level. (for research use only, not for use in clinical diagnostic procedures.)

For rapid, culture-free diagnostics, polymerase chain reaction (PCR) is an increasingly popular molecular technique.

Identification and susceptibility testing

PCR tools, such as Bruker’s Fungiplex® IVD assay solutions, allow clinicians to provide an unprecedented time-to-result in fungal infection cases. These real-time PCR assays ensure rapid, targeted results with high sensitivity and specificity, without relying on a lengthy culture process.

Due to the previous lack of specific identification methods, many fungal infections have been treated with broad-spectrum antimicrobial drugs, leading to MDR in some fungal species. Starting from a cultured sample, antimicrobial susceptibility testing (AST) can be run to establish whether the fungi in question, such as yeast or Cryptococci, are resistant to treatment.

Дополнительная информация

As of May 2021, Bruker Daltonik GmbH is now Bruker Daltonics GmbH & Co. KG.

Related products


Fungiplex® Aspergillus

The Fungiplex® Aspergillus IVD PCR Kit is a multiplex real-time PCR test for the most common pathogens associated with Invasive Aspergillosis.

Fungiplex® Aspergillus Azole-R

Fungiplex® Aspergillus Azole-R PCR IVD Kit is a real-time PCR test which identifies the most common mutations of the Cyp51 gene associated with resistance to the azole class of antifungal drugs.

Fungiplex® Candida

Fungiplex® Candida IVD PCR Kit is a multiplex real-time PCR assay, which rapidly detects the main causative species associated with Invasive Candidiasis.

Fungiplex® Candida Auris

Fungiplex® Candida Auris RUO PCR Kit is a real-time, research use only (RUO) PCR assay for the rapid detection of Candida auris in hospital hygiene applications.

Fungiplex® Universal

The Fungiplex® Universal Real-Time PCR RUO Kit is designed for research use only, to rapidly detect the presence of fungal DNA from samples without culture.

MBT Filamentous Fungi Library

MALDI Biotyper (MBT) provides high differentiation power for identification of filamentous fungi.


The increasing prevalence and severity of fungal infections in healthcare scenarios requires a standardized routine antifungal susceptibility test (AFST) in the microbiology lab. The MICRONAUT-AM system uses MIC plates for the automated or manual susceptibility testing of yeast and cryptococci.