Two-Photon Imaging of Freely Behaving Animals
Explore end-to-end workflows for two-photon imaging in freely behaving animals.
In this presentation, Dr. Waylin Yu (Bruker) introduces the nVista 2P and highlights the workflows supporting surgical procedures, data acquisition, processing, analysis, and sharing for two-photon imaging in freely behaving animals. He discusses system design, imaging performance, and tools for longitudinal cell tracking through the IDEAS platform.
Topics covered include:
- Design features that support freely behaving two-photon imaging and naturalistic movement.
- Advantages of 2P microscopy, including improved resolution, signal-to-noise ratio, and depth.
- Deep and shallow brain imaging using GRIN lenses and cranial windows.
- Workflows including motion correction, cell segmentation, event detection, and longitudinal cell registration.
- Integration of neural imaging data with behavioral and experimental measurements for downstream analysis and collaboration.
Two-Photon Imaging of Freely Behaving Animals
Inscopix aims to offer rapid and robust end-to-end data acquisition and processing capabilities through an easy-to-use product portfolio supporting surgical procedures, data acquisition, management, and analysis. Its latest development, the nVista 2P, is a plug-and-play system for free-behaving two-photon miniature microscope imaging. The nVista 2P has been validated for compatibility with GRIN lenses and cranial windows to enable deep and shallow brain imaging, as well as precise cell identification and registration over time. In this presentation, our lead application scientist, Dr. Waylin Yu, will highlight the user-friendly features of the Inscopix 2P workflow and the range of applications enabled by the nVista 2P and the Inscopix Data Exploration, Analysis & Sharing (IDEAS) platform.
Presented as part of our 2025 Online Neuroscience Summit — see all event information, speakers, and abstracts, or get instant on-demand access to this presentation and all other segments from the summit using the form below.
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Speaker
Waylin Yu, Ph.D.
Lead Application Scientist, Inscopix, a Bruker Company
