Bioimaging with Light-Sheet Microscopy: Ultra Gentle Imaging of Living Samples

Light-sheet microscopy is a state-of-the-art imaging technique that allows for long-term 3D imaging without the phototoxicity or photobleaching that occurs when using many standard imaging techniques like laser scanning confocal or spinning disk confocal.

Overview

In this webinar, we will discuss the benefits of light-sheet fluorescence microscopy as a gentle bio-imaging technique and show how it can be adapted to a variety of samples. Specifically, we will focus on the Luxendo InVi-SPIM with its unique light-sheet geometry, designed for imaging live specimens in high resolution, which until now have only been achieved using confocal microscopy. We will describe our innovative sample mounting technique and how we can use this to image cell lines, developing embryos, whole organisms and much more.

What to Expect

In this webinar, you can expect to learn the advantages of light-sheet fluorescence microscopy and how this technique can be adapted to a multitude of different samples and image these without the phototoxicity that plagues traditional imaging techniques. Expect to see comparisons of light sheet and spinning disk confocal microscopy.

Key Topics Covered:

Issues with Current Imaging Systems:

Balancing resolution and speed | Photobeaching | Phototoxicity during long time courses | The ability to image large volumes with sufficient time resolution

An Introduction to Light-sheet Microscopy:

What it is Light-Sheet Fluorescence Microscopy? | How Light-Sheet works | Luxendo Product Introduction -  InVi-SPIM

Applications:

Single particle tracking | Embryo development | Cellular imaging | IVF research | Neuroscience | Stem cell research | Bio-emitting organisms

 

 

 

 


About the Presenter

Dr. Dane Maxfield photo

Dr. Dane Maxfield graduated with a B.A in Mathematics and Biology from Concordia University- St. Paul, before going on to complete a master’s degree in Applied Mathematics and a PhD in Biology at the University of Utah. Dr Maxfield now holds the position of Luxendo Sales and Applications Specialist at Bruker Nano Surfaces.