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AFM in Conjunction with FluidFM® Technique and Applications

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Explore the advantages of the seamless combination of FluidFM with Bruker’s BioAFMs.

  • See exclusive presentations on the practical application of AFM in conjunction with FluidFM.
  • Gain new insight into the innovative, ongoing, and potential future research conducted with these technologies.
  • Understand the key considerations for the combined use of these technologies. 
  • Watch an expert-led technical demonstration of AFM-FluidFM instrument assembly, software configuration, and single-cell manipulation.

 

 

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Explore Advances in Stylus Profilometry

In this workshop, Accurate Metrology for Micro- and Nano-Fabrication with Stylus Profilometry, our speakers discuss key aspects of stylus profilometry, including the details researchers need to know to obtain reliable and repeatable results.

Particular attention is paid to the following topics:

  • What does a stylus profilometer measure?
  • How does a stylus profilometer work?
  • What tests, applications, and sample materials are stylus profilometers ideally suited for?
  • How do you select the right stylus probe for your application?

 

If you have any questions about our products or services, please contact us. Follow @BrukerNano on Twitter for event, product, and webinar updates.

Discover the fascinating capabilities of combining Bruker’s BioAFM techniques with the Cytosurge FluidFM technology.

Featuring special guest speakers alongside a panel of Bruker application experts from across Europe, the program includes both detailed presentations and live demonstrations highlighting the features, capabilities, and practical use of these tools. Attendees can expect to gain new insight into the novel experimental designs and applications made possible by combining FluidFM with Bruker’s BioAFMs.

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For more information about this event or the products and techniques featured in it, please contact us.

Recorded Tuesday, October 12, 2021

Combining Atomic Force Microscopy and Nanofluidics

How FluidFM technology enhances the versatility of Bruker BioAFMs

Atomic Force Microscopy (AFM) is an advanced multi-parametric imaging technique that enables the 3D imaging of the topography of biological samples in the nm-range, the characterization of their nanomechanical properties, and the visualization of interactions and structural changes occurring at the molecular level. 

The FluidFM (Fluidic Force Microscopy) technology features microchanneled, force-sensing cantilevers with microfluidic pressure control. They enable the handling of femtoliter volumes, precise pressure control (0.1mBar), and can be used as nano-syringes to extract cell material or inject fluids into singles cells or tissues.

The seamless combination of FluidFM with Bruker’s BioAFMs opens the door to a host of novel experiment designs and applications. It enables a broader range of force control, the use of microfluidics for single-cell manipulation applications, and accurate positioning combined with perfect optical integration for observation and control.

Applications include:

  1. Single-cell injection or extraction
  2. Single-cell level adhesion measurements with forces >50nN
  3. Enhanced nanomanipulation capabilities
  4. Trigger-Tracking experiments: Precise localized injection into cells (FluidFM) and tracking of interactive forces, topographic changes, or nanomechanical properties (AFM)
  5. Access to single-cell gene editing, injection of targeted drugs or gene vectors for AFM users
Recorded on October 12, 2021

Featured Presentations

30 Minutes | Developing New and Efficient Methods for Harvesting Microalgae using the AFM and FluidFM Technologies

Cécile Formosa-Dague, Ph.D., CNRS Researcher
Toulouse Biotechnology Institute (TBI), INSA de Toulouse, France

Dr. Cécile Formosa-Dague, Ph.D., TBI, INSA de Toulouse, will speak on her work developing novel harvesting methods for microalgae, a promising resource for biofuel production, using the AFM and FluidFM Technologies.

15 Minutes | FluidFM Application Highlights


Mirko Vanetti, Ph.D., Cytosurge AG, Switzerland

Dr. Mirko Vanetti, Cytosurge AG, will speak on the growing field of applications for the FluidFM technology, and outline examples of how the technology can support CRISPR, neurobiology, and virus research.

10 Minutes | NanoWizard AFM Family in Conjunction with FluidFM

Torsten Mueller, Ph.D., Senior Developer Life Sciences, Bruker
André Körnig, Ph.D., Applications Scientist, Bruker

 

10 Minutes | Live Demonstration: BioAFM-FluidFM

André Körnig, Ph.D., Applications Scientist, Bruker

Speakers


With introduction and closing remarks by Carmen Pettersson, Marcom Manager EMEA, Bruker BioAFM

Cécile Formosa-Dague, Ph.D., CNRS Researcher, Toulouse Biotechnology Institute (TBI), INSA de Toulouse, France

Dr. Cécile Formosa-Dague did her PhD between 2012 and 2015 at the Laboratory for Analysis and Architecture of the Systems (LAAS-CNRS) in Toulouse, where she worked on important issues related to multidrug-resistant microorganisms using AFM. After that, she joined the Y. Dufrêne team at the Catholic University of Louvain (UCL) in Belgium, where she pushed further the technological concepts developed during her PhD to study biofilm formation in bacteria. In 2017, after having won a Marie Curie grant, she moved to the TBI (Toulouse Biotechnology Institute) where she worked on developing interdisciplinary approaches to studying microalgae and their interactions with their environment using AFM techniques. Finally, in 2019, she obtained a permanent position at the Centre National de la Recherche Scientifique (CNRS) where she continues to develop these research activities on microalgae, at the TBI, and to work on new techniques based on the FluidFM technology.

Mirko Vanetti, Ph.D., Cytosurge AG, Switzerland