In Episode 2 of The Light-Sheet Chronicles Podcast podcast, host Dr. Elisabeth Kugler meets with special guests Dr. Urmas Roostalu (Gubra) and Dr. Johanna Perens (Gubra) and Bruker light-sheet microscopy expert Dr. Malte Wachsmuth to discuss applications from subcellular dynamics to whole-organ imaging, highlighting techniques like in vivo imaging, tissue clearing, and data analysis.
They address challenges such as data size, image tiling, and quality control. A standout moment features the "Doritos paper," showcasing how a food dye enables temporary tissue transparency in live animals.